Garcia-Cruz A, Ahmad O S, Alanazi K, Piletska E, Piletsky S A
School of Chemistry, University of Leicester, Leicester, UK.
Microsyst Nanoeng. 2020 Oct 19;6:83. doi: 10.1038/s41378-020-00193-3. eCollection 2020.
The present research describes the design of robust electrochemical sensors based on electro-responsive molecularly imprinted polymer nanoparticles (e-MIPs). The e-MIPs, tagged with a redox probe, combine both recognition and reporting functions. This system replaces enzyme-mediator pairs used in traditional biosensors. The analyte recognition process relies on the generic actuation phenomenon when the polymer conformation of e-MIPs is changing in response to the presence of the template analyte. The analyte concentration is measured using voltammetric methods. In an exemplification of this technology, electrochemical sensors were developed for the determination of concentrations of trypsin, glucose, paracetamol, C4-homoserine lactone, and THC. The present technology allows for the possibility of producing generic, inexpensive, and robust disposable sensors for clinical, environmental, and forensic applications.
本研究描述了基于电响应分子印迹聚合物纳米颗粒（e-MIPs）的稳健电化学传感器的设计。带有氧化还原探针的e-MIPs结合了识别和报告功能。该系统取代了传统生物传感器中使用的酶-介体对。分析物识别过程依赖于当e-MIPs的聚合物构象响应模板分析物的存在而发生变化时的一般驱动现象。使用伏安法测量分析物浓度。作为该技术的一个示例，开发了用于测定胰蛋白酶、葡萄糖、对乙酰氨基酚、C4-高丝氨酸内酯和四氢大麻酚浓度的电化学传感器。本技术使得有可能生产出用于临床、环境和法医应用的通用、廉价且稳健的一次性传感器。
