Ali Zaima, Khaliq Saba, Zaki Saima, Ahmad Hafiz Usman, Lone Khalid Pervaiz
Department of Physiology and Cell Biology, University of Health Sciences Lahore, Lahore, Pakistan.
Department of Physiology, Lahore Medical and Dental College Lahore, Lahore, Pakistan.
Int J Reprod Biomed. 2020 Apr 30;18(4):235-242. doi: 10.18502/ijrm.v13i4.6886. eCollection 2020 Apr.
Preeclampsia is a hypertensive disorder of pregnancy affecting about 2-10% pregnancies worldwide. mRNA expression of tumor necrosis factor alpha and have been reported to be altered in placental bed in preeclamptic pregnancies. We hypothesized that the expression of these genes is also altered in peripheral blood mononuclear cells (PBMCs) in preeclampsia.
To compare the expression of Fas receptor and related genes in PBMCs of preeclamptic and normotensive pregnant women.
A cross-sectional comparative study comprising of 18 cases and 18 controls was designed. 5 ml of venous blood was drawn and collected considering aseptic measures. Buffy coat was separated by centrifugation and stored at -20°C. Favor Prep total RNA Isolation Kit (Favorgen, Taiwan) was used for RNA extraction. The mRNA expression of and was measured by real-time polymerase chain reaction in PBMCs in preeclamptic and normal pregnancies.
A significant increase in mRNA expression of and (p 0.001) was observed in PBMCs of preeclamptic pregnancies compared to the control group (p 0.001). Moreover, a significant positive correlation was found between the mRNA expression and and (p 0.001).
The results lead to the conclusion that mRNA expression of and in the maternal PBMCs is altered in preeclamptic pregnancies and might contribute to the pathogenesis of the disease.
子痫前期是一种妊娠期高血压疾病，在全球约2% - 10%的妊娠中出现。据报道，肿瘤坏死因子α的mRNA表达在子痫前期妊娠的胎盘床中发生改变。我们推测这些基因的表达在子痫前期患者的外周血单个核细胞（PBMCs）中也有改变。
比较子痫前期孕妇和血压正常孕妇PBMCs中Fas受体及相关基因的表达。
设计了一项包含18例病例和18例对照的横断面比较研究。采取无菌措施抽取5ml静脉血。通过离心分离出白细胞层并储存在-20°C。使用Favor Prep总RNA分离试剂盒（台湾Favorgen公司）进行RNA提取。通过实时聚合酶链反应测量子痫前期妊娠和正常妊娠PBMCs中 和 的mRNA表达。
与对照组相比，子痫前期妊娠PBMCs中 和 的mRNA表达显著增加（p < 0.001）。此外， mRNA表达与 和 之间存在显著正相关（p < 0.001）。
结果表明，子痫前期妊娠母体PBMCs中 和 的mRNA表达发生改变，可能参与了该疾病的发病机制。
