Department of Bioinformatics and biostatistics, National University Biomedical Research Institute, National University-Sudan, Khartoum- Sudan.
Department microbiology University of Alrazi-Khartoum- Sudan.
Int J Infect Dis. 2020 Aug;97:126-130. doi: 10.1016/j.ijid.2020.05.101. Epub 2020 Jun 1.
The aim of this study was to determine the OBI in plasma and urine samples from renal transplant patients using Multiplex Nested PCR.
A total of 100 samples (plasma and urine) were collected from renal transplant patients admitted to the renal transplant center in Khartoum north, Sudan in 2019. For each sample, HBsAg, HBeAg and anti HBcAg were detected using Enzyme linked Immune sorbent assay (ELISA). The viral DNA was then extracted using viral DNA extraction kit and were then tested for HBV DNA by using multiplex nested PCR. Statistical analysis was done using statistical package of social science (IBM SPSS version 20.0) considering a P value ≤ 0.05 as a level of significance.
HBsAg were not detected in al patient but, HBeAg were 14 (14%) and anti HBcAg were 36 (36%)were detect by using ELISA. A total 18 (18%) and 3 out of 100 were found positive in plasma and urine samples, respectively. Regarding the virus genotypes, D, E and mixed D/E genotypes were detected in all positive samples. Females were significantly (P value=0.013) higher detectable with HBV than males in plasma samples CONCLUSION: OBI incidence in renal transplant patients is high in Sudan. The multiplex nested PCR had identified OBI with a high rate supporting the efficiency of using molecular techniques in detecting of HBV. This will lead to an appropriate diagnosis and minimizing the risk to be infected by HBV.
本研究旨在使用多重巢式 PCR 来确定肾移植患者的血液和尿液样本中的 OBI。
2019 年，从苏丹喀土穆北部的肾移植中心采集了 100 例肾移植患者的血液和尿液样本。对每个样本，使用酶联免疫吸附测定法（ELISA）检测 HBsAg、HBeAg 和抗 HBcAg。然后使用病毒 DNA 提取试剂盒提取病毒 DNA，并使用多重巢式 PCR 检测 HBV DNA。使用社会科学统计软件包（IBM SPSS 版本 20.0）进行统计分析，以 P 值≤0.05 为显著性水平。
所有患者均未检测到 HBsAg，但 14 例（14%）患者检测到 HBeAg，36 例（36%）患者检测到抗 HBcAg。总共在 18 例（18%）患者的血液和 3 例（3%）患者的尿液样本中发现阳性。关于病毒基因型，所有阳性样本中均检测到 D、E 和混合 D/E 基因型。在血液样本中，女性比男性更能显著（P 值=0.013）检测到 HBV。
在苏丹，肾移植患者的 OBI 发病率较高。多重巢式 PCR 已确定 OBI 的高发生率，支持使用分子技术检测 HBV 的效率。这将有助于进行适当的诊断并最大限度地降低感染 HBV 的风险。
