School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510006, China.
College of Mathematics and Statistics, Shenzhen University, Shenzhen, Guangdong, 518060, China.
Biomed Pharmacother. 2020 Aug;128:110270. doi: 10.1016/j.biopha.2020.110270. Epub 2020 Jun 2.
The pathogenesis of deep vein thrombosis (DVT) is incompletely understood, requiring reliable animal models. Inferior vena cava (IVC) stenosis model mimics human DVT.
To provide optimal conditions for establishing a rat model of IVC stenosis-induced DVT.
Effects of suture, and body weight, sex and side branches of rats on the IVC stenosis model were evaluated. 1 d after modeling, the weight and length of thrombosed IVCs and side branch distance were measured. Histopathological change and leukocytes influxes were observed by hematoxylin and eosin staining. Ly-6G-positive neutrophils were located by immunofluorescence. A multiple regression linear model was then built.
IVCs stenosed with silk or monofilament sutures presented no difference in leukocyte influxes. Thrombus of 220-340 g rats was significantly heavier than that of 180-220 g rats. Although no statistic difference was found in thrombus weight between male and female rats weighing 180-260 g, males weighing 260-300 g formed larger thrombi than weight-matched females. Thrombus weight and length of rats except 180-220 g females was not impacted by side branch ligation and side branch distance. The regression model showed that sex and body weight were key factors affecting thrombus weight.
Male and female rats weighing 220-260 g are more suitable for establishing a model of DVT induced by stenosing IVC with silk and without side branch ligation.
深静脉血栓形成（DVT）的发病机制尚不完全清楚，需要可靠的动物模型。下腔静脉（IVC）狭窄模型模拟人类 DVT。
为建立 IVC 狭窄诱导 DVT 的大鼠模型提供最佳条件。
评估缝线、大鼠体重、性别和侧支对 IVC 狭窄模型的影响。建模后 1 天，测量血栓形成的 IVC 和侧支距离的重量和长度。通过苏木精和伊红染色观察组织病理学变化和白细胞浸润。通过免疫荧光定位 Ly-6G 阳性中性粒细胞。然后建立了一个多元回归线性模型。
用丝线或单丝缝线狭窄的 IVC 白细胞浸润无差异。220-340g 大鼠的血栓明显比 180-220g 大鼠的重。虽然 180-260g 体重的雄性和雌性大鼠的血栓重量没有统计学差异，但 260-300g 体重的雄性大鼠形成的血栓比体重匹配的雌性大鼠大。除了 180-220g 雌性大鼠之外，侧支结扎和侧支距离对大鼠的血栓重量和长度没有影响。回归模型显示，性别和体重是影响血栓重量的关键因素。
体重为 220-260g 的雄性和雌性大鼠更适合建立用丝线且不结扎侧支的 IVC 狭窄诱导 DVT 模型。
