Zhang Hui, Liu Yating, Hu Fengxian, Chen Siyi, Xie Zhoutao, Wu Hezhen, Zhang Ting, Liu Bo
School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China.
Key Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province, Wuhan, China.
PLoS One. 2025 Apr 30;20(4):e0321023. doi: 10.1371/journal.pone.0321023. eCollection 2025.
Justicia procumbens L. contains lignans with remarkable antiplatelet aggregation effects. Its ethyl acetate extract has demonstrated the efficacy of inhibiting platelet aggregation in preliminary tests. Identifying key components, elucidating the antithrombotic mechanism, and evaluating its potential for treating thrombotic diseases are of great significance.
Chemical analysis was conducted on the ethyl acetate extract of J. procumbens. Animal models of carotid artery and venous thrombosis, combined with proteomics analysis, were employed to study the antithrombotic mechanism.
Chemical analysis identified justicidin B (JB) and chinensinaphthol methyl ether (CME) as the major active antithrombotic constituents. The extract inhibited platelet aggregation by regulating relevant signaling pathways, showing potential in preventing both venous and arterial thrombosis.
This study confirmed that the ethyl acetate extract of J. procumbens, especially the lignan components JB and CME, can effectively inhibit platelet aggregation, showing promising prospects in treating thrombotic diseases. This provides ideas for the development of health products based on J. procumbens and lays a foundation for further clinical exploration.
爵床含有具有显著抗血小板聚集作用的木脂素。其乙酸乙酯提取物在初步试验中已显示出抑制血小板聚集的功效。确定关键成分、阐明抗血栓形成机制并评估其治疗血栓性疾病的潜力具有重要意义。
对爵床的乙酸乙酯提取物进行化学分析。采用颈动脉和静脉血栓形成动物模型，并结合蛋白质组学分析来研究抗血栓形成机制。
化学分析确定了爵床定B（JB）和中国萘酚甲醚（CME）为主要的抗血栓活性成分。该提取物通过调节相关信号通路抑制血小板聚集，在预防静脉和动脉血栓形成方面均显示出潜力。
本研究证实爵床的乙酸乙酯提取物，尤其是木脂素成分JB和CME，可有效抑制血小板聚集，在治疗血栓性疾病方面显示出广阔前景。这为基于爵床开发保健品提供了思路，并为进一步的临床探索奠定了基础。
