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给饲喂青贮冷季牧草的肉牛测定表观全肠道消化率、瘤胃发酵和血液代谢物。

Apparent total tract digestibility, ruminal fermentation, and blood metabolites in beef steers fed green-chopped cool-season forages.

机构信息

Department of Animal Sciences, North Florida Research and Education Center, University of Florida, Marianna, FL.

Agronomy Department, North Florida Research and Education Center, University of Florida, Marianna, FL.

出版信息

J Anim Sci. 2020 Jun 1;98(6). doi: 10.1093/jas/skaa175.

Abstract

An experiment was conducted during the winter of two consecutive years to evaluate the effects of feeding green-chopped cool-season forages on digestibility, ruminal fermentation, and blood parameters in beef steers. Nine ruminally cannulated Angus crossbred steers (year 1: 359 ± 79 kg; year 2: 481 ± 105 kg) received ad libitum green-chopped forages from pastures planted with one of the following mixtures: 1) OAT = Horizon 201 oats (Avena sativa L.)/Prine annual ryegrass (Lolium multiflorum Lam.) at 95 and 17 kg/ha, respectively; 2) RYE = FL401 cereal rye (Secale cereale L.)/Prine annual ryegrass (Lolium multiflorum Lam.) at 78 and 17 kg/ha, respectively; or 3) TRIT = Trical 342 triticale (X Triticosecale spp.)/Prine annual ryegrass (Lolium multiflorum Lam.) at 95 and 17 kg/ha, respectively. Intake was measured using the GrowSafe system and orts were discarded prior to subsequent feeding. After a 14-d adaptation, feed and fecal samples were collected twice daily for 4 d to determine apparent total tract nutrient digestibility using indigestible neutral detergent fiber (NDF) as an internal marker. On day 19, blood and ruminal fluid samples were collected every 3 h during a 24-h period to analyze plasma urea nitrogen (PUN) and glucose, ruminal pH, and concentration of ruminal ammonia nitrogen (NH3-N) and volatile fatty acids (VFA). Data were analyzed as a generalized randomized block design with repeated measures using the PROC MIX of SAS. No effect of treatment (P > 0.05) was observed for intake of dry matter, organic matter (OM), crude protein, NDF, or acid detergent fiber. Apparent total tract digestibility of nutrients was greater (P < 0.05) for OAT and TRIT when compared with RYE, with OM digestibility being 82.7%, 79.6%, and 69.5%, respectively. An effect of time (P < 0.01) was observed for ruminal pH. Plasma concentration of glucose was greater (P < 0.01) in steers consuming OAT, whereas steers fed RYE had greater (P < 0.05) concentrations of ruminal NH3-N and PUN, and the least concentration of total ruminal VFA (P < 0.05), despite having the greatest (P > 0.05) molar proportion of acetate, branched-chain VFA, and acetate:propionate. Increased nutrient digestibility and favorable ruminal fermentation and blood metabolites of OAT and TRIT are potentially conducive to enhanced growth performance when compared with RYE.

摘要

在连续两年的冬季进行了一项实验,以评估喂养绿色切碎的冷季饲草对肉牛干物质采食量、瘤胃发酵和血液参数的影响。9 头瘤胃切开的安格斯杂交公牛(第 1 年:359±79kg;第 2 年:481±105kg)自由采食来自种植以下混合物之一的牧场的绿色切碎饲草:1)OAT = Horizon 201 燕麦(Avena sativa L.)/普赖恩一年生黑麦草(Lolium multiflorum Lam.),分别为 95 和 17kg/ha;2)RYE = FL401 黑麦(Secale cereale L.)/普赖恩一年生黑麦草(Lolium multiflorum Lam.),分别为 78 和 17kg/ha;或 3)TRIT = Trical 342 小黑麦(X Triticosecale spp.)/普赖恩一年生黑麦草(Lolium multiflorum Lam.),分别为 95 和 17kg/ha。采用 GrowSafe 系统测量采食量,在随后的喂养前丢弃残渣。适应期 14d 后,每天两次收集 4d 的饲料和粪便样品,用不可消化的中性洗涤纤维(NDF)作为内标物测定表观全肠道养分消化率。在第 19 天,在 24h 期间每隔 3h 采集一次血液和瘤胃液样品,分析血浆尿素氮(PUN)和葡萄糖、瘤胃 pH 值以及瘤胃氨氮(NH3-N)和挥发性脂肪酸(VFA)浓度。数据采用 SAS 中的 PROC MIX 作为广义随机区组设计进行分析,采用重复测量。处理(P>0.05)对干物质、有机物(OM)、粗蛋白、NDF 或酸性洗涤剂纤维的采食量没有影响。与 RYE 相比,OAT 和 TRIT 的养分表观全肠道消化率更高(P<0.05),OM 消化率分别为 82.7%、79.6%和 69.5%。瘤胃 pH 值随时间(P<0.01)而变化。OAT 组牛的血浆葡萄糖浓度更高(P<0.01),而 RYE 组牛的瘤胃 NH3-N 和 PUN 浓度更高(P<0.05),总瘤胃 VFA 浓度最低(P<0.05),尽管乙酸、支链 VFA 和乙酸:丙酸的摩尔比例最高(P>0.05)。OAT 和 TRIT 的养分消化率提高、瘤胃发酵和血液代谢物良好,与 RYE 相比,可能有利于提高生长性能。

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