一种应用于模板胆固醇的新型分子印迹方法。

A novel method of molecular imprinting applied to the template cholesterol.

机构信息

University of Belgrade - Faculty of Chemistry, Studentski trg 12-16, 11000, Belgrade, Serbia.

Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szent Gellert ter 4, H-1111, Budapest, Hungary.

出版信息

Talanta. 2020 Sep 1;217:121075. doi: 10.1016/j.talanta.2020.121075. Epub 2020 Apr 23.

DOI:10.1016/j.talanta.2020.121075

PMID:32498845

Abstract

A novel method is successfully tested for non-covalent imprinting. Conditions are used which practically exclude the formation of prepolymerization complexes. The template is cholesterol, and no so-called functional monomer is used. The polymers contain only an acrylic diester crosslinker. The porogen isopropanol prevents even hydrogen bonding between the template and the monomer in the prepolymerization solution. Despite of these apparently very disadvantageous conditions, appreciable imprinting factors for cholesterol and imprinted selectivity against some other steroids are observed, similar to other cholesterol MIPs with proven analytical usefulness.

摘要

一种新的方法被成功地应用于非共价印迹。所采用的条件实际上排除了预聚合复合物的形成。模板为胆固醇，且不使用所谓的功能单体。聚合物仅含有一种丙烯酸双酯交联剂。致孔剂异丙醇甚至阻止了模板和单体在预聚合溶液中的氢键形成。尽管这些条件明显非常不利，但仍观察到了对胆固醇的显著印迹因子和对其他一些甾体的印迹选择性，类似于其他具有证明的分析有用性的胆固醇 MIP。