Polymeric Materials Area, Chemical and Material Sciences Division, CSIR-Indian Institute of Petroleum, Dehradun 248005, India.
Department of Chemical Science, Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Molecules. 2022 Apr 30;27(9):2856. doi: 10.3390/molecules27092856.
Sensitive and rapid monitoring of cholesterol levels in the human body are highly desirable as they are directly related to the diagnosis of cardiovascular diseases. By using the nanoarchitectonic approach, a novel fluorescent conjugated oligofluorene (OFP-CD) functionalized with β-cyclodextrin (β-CD) was assembled for "Turn-On" fluorescence sensing of cholesterol. The appended β-CD units in OFP-CD enabled the forming of host-guest complexes with dabsyl chloride moieties in water, resulting in fluorescence quenching of the oligofluorene through intermolecular energy transfer. In the presence of cholesterol molecules, a more favorable host-guest complex with stoichiometry 1 cholesterol: 2 β-CD units was formed, replacing dabsyl chloride in β-CD's cavities. This process resulted in fluorescence recovery of OFP-CD, owing to disruption of energy transfer. The potential of this nanoarchitectonic system for "Turn-On" sensing of cholesterol was extensively studied by fluorescence spectroscopy. The high selectivity of the sensor for cholesterol was demonstrated using biologically relevant interfering compounds, such as carbohydrates, amino acids, metal ions, and anions. The detection limit (LOD value) was as low as 68 nM, affirming the high sensitivity of the current system.
灵敏、快速监测人体胆固醇水平非常重要，因为它与心血管疾病的诊断直接相关。通过使用纳米结构方法，组装了一种新型的荧光共轭寡聚芴（OFP-CD），并用β-环糊精（β-CD）进行功能化，用于胆固醇的“开启”荧光传感。OFP-CD 中的附加β-CD 单元能够在水中与二甲氨基萘酰氯部分形成主客体配合物，通过分子间能量转移导致寡聚芴的荧光猝灭。在胆固醇分子存在下，形成了具有化学计量比 1：2 的更有利的主客体配合物，取代了β-CD 腔中的二甲氨基萘酰氯。这个过程导致 OFP-CD 的荧光恢复，这是由于能量转移的中断。通过荧光光谱法广泛研究了这种纳米结构体系对胆固醇的“开启”传感的潜力。该传感器对胆固醇具有高选择性，使用了具有生物学相关性的干扰化合物，如碳水化合物、氨基酸、金属离子和阴离子。检测限（LOD 值）低至 68 nM，证实了当前系统的高灵敏度。
