Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, China.
College of Life and Health Sciences, Northeastern University, Shenyang, 110169, China.
Talanta. 2020 Sep 1;217:121014. doi: 10.1016/j.talanta.2020.121014. Epub 2020 Apr 11.
A porphyrin structure carbon dots (CDs) with red fluorescence is prepared by the reaction of terephthalaldehyde with pyrrole under the trifluoroacetic acid catalyst. The CDs with an average particle size of 5.70 nm are red-emission with a maximum fluorescent wavelength at 650 nm due to the large conjugate structure of the porphyrin ring, giving rise to a quantum yield of 14%. The aldehyde groups in CDs make self-aggregation possible, and promote the effective coating of CDs around Transferrin (Trf) via the Schiff base reaction, forming CDs@Trf nanocomposites with a particle size of 19.7 nm. Trf can specifically target cells with Trf receptors on the surface of cell membrane. CDs@Trf exhibits stable red-emission in low polarity solvents, and are able to generate singlet oxygen (O), which facilitates cell imaging and photodynamic therapy. In vitro cell experiments demonstrate that CDs@Trf greatly inhibits the growth of tumor cells under laser irradiation at 635 nm. After incubation of 3 mg mL CDs@Trf with Bend.3 cells, the survival rate of the cells is only 26% under laser irradiation of 4.78 mW cm for 5 min.
一种具有红色荧光的卟啉结构碳点（CDs）是通过三氟乙酸催化剂下的对苯二甲醛与吡咯反应制备的。由于卟啉环的大共轭结构，CDs 的平均粒径为 5.70nm，具有最大荧光波长为 650nm 的红色发射，量子产率为 14%。CDs 中的醛基使其能够发生自聚集，并通过希夫碱反应促进 CDs 有效地包裹转铁蛋白（Trf），形成粒径为 19.7nm 的 CDs@Trf 纳米复合材料。Trf 可以特异性地靶向细胞膜表面具有 Trf 受体的细胞。CDs@Trf 在低极性溶剂中表现出稳定的红色发射，并能够产生单线态氧（O），这有利于细胞成像和光动力治疗。体外细胞实验表明，CDs@Trf 在 635nm 激光照射下能极大地抑制肿瘤细胞的生长。在与 Bend.3 细胞孵育 3mg mL CDs@Trf 后，在 4.78mW cm 的激光照射下 5min 后，细胞的存活率仅为 26%。
