College of Chemistry and Chemical Engineering, North Minzu University, Yinchuan, 750021, China; CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Talanta. 2020 Sep 1;217:121082. doi: 10.1016/j.talanta.2020.121082. Epub 2020 Apr 26.
Hydrophilic interaction chromatography (HILIC), as one of the important methods for glycopeptides enrichment, has attracted the attention of more and more researchers due to the high efficiency in glycopeptides enrichment. Ordered mesoporous silica materials have been used in many fields, such as catalysis, separation and drug delivery, owing to their adjustable pore size, excellent mechanical properties and ease of preparation. In our case, a series of ordered mesoporous silicas with different pore sizes (3.7-16.7 nm) were prepared via sol-gel reaction employing F127 as the template and TEOS as the silica source. After modified with glutathione by photo-initiated thiol-ene reaction, these materials exhibited hydrophilicity and could be used as adsorbents of HILIC for capturing N-linked glycopeptides from IgG and serum protein tryptic digests. Up to 26 N-glycopeptides were identified from IgG digest, and 565 N-glycopeptides and 330 N-glycosylation sites, mapped to 159 glycoproteins, were identified from 2 μL human serum digest, indicating the great ability for enriching N-glycopeptides from complex biological samples.
亲水作用色谱（HILIC）作为一种重要的糖肽富集方法，由于其在糖肽富集方面的高效性，引起了越来越多研究人员的关注。有序介孔硅材料由于其可调的孔径、优异的机械性能和易于制备，已被广泛应用于催化、分离和药物输送等领域。在我们的研究中，通过溶胶-凝胶反应，以 F127 为模板剂，TEOS 为硅源，制备了一系列具有不同孔径（3.7-16.7nm）的有序介孔硅。然后通过光引发的硫醇-烯反应对其进行谷胱甘肽改性，这些材料表现出亲水性，可作为 HILIC 的吸附剂，用于从 IgG 和血清蛋白酶解物中捕获 N-连接的糖肽。从 IgG 酶解物中鉴定出了 26 个 N-糖肽，从 2μL 人血清酶解物中鉴定出了 565 个 N-糖肽和 330 个 N-糖基化位点，映射到 159 个糖蛋白，表明其从复杂生物样品中富集 N-糖肽的能力很强。
