Hart C A, Batt R M, Saunders J R, Getty B
Dept. of Medical Microbiology, University of Liverpool, U.K.
Scand J Gastroenterol. 1988 Dec;23(10):1153-9. doi: 10.3109/00365528809090184.
The ultrastructural effects of 11 lectins on the intestinal brush border were examined by means of an in vitro rabbit ileal mucosal explant culture system. Five of the lectins that bind to oligosaccharides containing either N-acetylglucosamine (phytohaemagglutinin, Euonymus europaeus lectin, pokeweed mitogen, and wheat germ agglutinin) or N-acetylneuraminic acid (Mycoplasma gallisepticum lectin) all had a specific effect on microvilli. The effects varied in accordance with the lectin and included lengthening, distortion, and vesiculation of the microvilli. In contrast, lectins binding specifically to galactose, glucose, mannose, and N-acetylgalactosamine had no effect. Incubation of mucosal fragments with the divalent cation ionophore A23187 did not mimic the effect of the lectins. This apparent relationship between lectin damage and receptor specificity may reflect either accessibility of appropriate binding sites or a differential response to binding.
利用体外兔回肠黏膜外植体培养系统，研究了11种凝集素对肠刷状缘的超微结构影响。其中5种凝集素可与含有N-乙酰葡糖胺（植物血凝素、卫矛凝集素、商陆促有丝分裂原和麦胚凝集素）或N-乙酰神经氨酸（鸡败血支原体凝集素）的寡糖结合，它们均对微绒毛有特异性作用。这些作用因凝集素而异，包括微绒毛的延长、变形和形成囊泡。相比之下，特异性结合半乳糖、葡萄糖、甘露糖和N-乙酰半乳糖胺的凝集素则无作用。用二价阳离子载体A23187孵育黏膜片段并不能模拟凝集素的作用。凝集素损伤与受体特异性之间的这种明显关系可能反映了合适结合位点的可及性或对结合的不同反应。
