Centre for Natural Products & Traditional Knowledge, CSIR Indian Institute of Chemical Technology, Hyderabad-500007, India.
Academy of Scientific & Innovative Research, CSIR Indian Institute of Chemical Technology, Hyderabad-500007, India and Department of Analytical & Structural Chemistry, CSIR Indian Institute of Chemical Technology, Hyderabad-500007, India.
Food Funct. 2020 Jun 24;11(6):5486-5497. doi: 10.1039/c9fo02714a.
Non-enzymatic reactions between proteins and methylglyoxal (MG) result in the formation of advanced glycation end products (AGEs). These AGEs play a vital role in the development of diabetic complications by stimulating oxidative stress and acting upon their receptor RAGE (Receptor for Advanced Glycation End products). This study examined the effect of aqueous methanol extract of Bombax ceiba L. calyxes (BCCE) on MG induced protein glycation and oxidative stress, followed by the identification of phytometabolites present in the calyxes using gas chromatography-mass spectrometry (GC-MS). The study revealed that priming of bovine serum albumin protein with the BCCE inhibited MG induced AGE formation in vitro and restrained AGE-induced RAGE up-regulation in HEK-293 cells. The BCCE significantly (p < 0.001) reduced the MG induced increase in reactive oxygen species (ROS), NADPH oxidase (NOX), and mitochondrial dysfunction. Improvements in the levels of antioxidant enzymes such as Mn and Cu/Zn-superoxide dismutase and glutathione reductase were also observed in HEK-293 cells. Furthermore, the decrease in primary cellular defense against AGEs, the glyoxalase 1 (Glo-1) activity, due to MG treatment was restored in BCCE treated cells. GC-MS analysis revealed the presence of antioxidant and antiglycation compounds such as myo-ionisitol, scopoletin, d-sedoheptulose, succinic acid, and xylitol in B. ceiba calyxes. The observed beneficial effect in our study might be attributed to the presence of these compounds in B. Ceiba calyxes. This is the first report presenting the antioxidant and antiglycation activities of B. ceiba calyxes and GC-MS analysis of active phytometabolites. These observations show that B. ceiba calyxes may become a potent and promising functional food to manage/control the development of diabetic complications.
蛋白质与甲基乙二醛（MG）之间的非酶反应导致晚期糖基化终产物（AGEs）的形成。这些 AGEs 通过刺激氧化应激并作用于其受体 RAGE（晚期糖基化终产物受体），在糖尿病并发症的发展中起着至关重要的作用。本研究检查了 Bombax ceiba L. 花萼的水甲醇提取物（BCCE）对 MG 诱导的蛋白质糖基化和氧化应激的影响，然后使用气相色谱-质谱联用（GC-MS）鉴定花萼中存在的植物代谢物。研究表明，用 BCCE 对牛血清白蛋白蛋白进行预处理可抑制体外 MG 诱导的 AGE 形成，并抑制 HEK-293 细胞中 AGE 诱导的 RAGE 上调。BCCE 可显著（p < 0.001）降低 MG 诱导的活性氧（ROS）、NADPH 氧化酶（NOX）和线粒体功能障碍的增加。还观察到 HEK-293 细胞中抗氧化酶如 Mn 和 Cu/Zn-超氧化物歧化酶和谷胱甘肽还原酶水平的提高。此外，由于 MG 处理，对 AGEs 的初级细胞防御作用下降，即糖氧还蛋白 1（Glo-1）活性，在 BCCE 处理的细胞中得到恢复。GC-MS 分析显示，B. ceiba 花萼中存在抗氧化和抗糖化化合物，如肌醇、东莨菪素、d-山梨糖醇、琥珀酸和木糖醇。我们的研究中观察到的有益作用可能归因于 B. Ceiba 花萼中存在这些化合物。这是首次报道 B. ceiba 花萼的抗氧化和抗糖化活性以及活性植物代谢物的 GC-MS 分析。这些观察结果表明，B. ceiba 花萼可能成为一种有潜力的功能性食品，以管理/控制糖尿病并发症的发展。
