Vels Institute of Science, Technology & Advanced Studies, Chennai, India.
Int J Low Extrem Wounds. 2021 Dec;20(4):364-373. doi: 10.1177/1534734620921629. Epub 2020 Jun 5.
Full-thickness burns pose a major challenge for clinicians to handle because of their restricted self-healing ability. Even though several approaches have been implemented for repairing these burnt skin tissue defects, all of them had unsatisfactory outcomes. Moreover, during recent years, skin tissue engineering techniques have emerged as a promising approach to improve skin tissue regeneration and overcome the shortcomings of the traditional approaches. Although previous literatures report the wound healing effects of the squalene oil, in the current study, for the first time, we developed a squalene-loaded emulgel-based scaffold as a novel approach for potential skin regeneration. This squalene-loaded agar-based emulgel scaffold was fabricated by using physical cross-linking technique using lecithin as an emulsifier. Characterization studies such as X-ray diffraction, Fourier-transform infrared spectroscopy, and field emission scanning electron microscopy revealed the amorphous nature, chemical interactions, and cross-linked capabilities of the developed emulgel scaffold. The squalene-loaded emulgel scaffold showed excellent wound contraction when compared with the agar gel and negative control. In case of the histopathology and recent immunohistochemistry findings, it was clearly evidenced that squalene-loaded emulgel promoted faster rate of the revascularization and macrophage polarization in order to enhance the burn wound healing. Moreover, the findings also revealed that the incorporation of squalene oil into the formulation enhances collagen deposition and accelerates the burnt skin tissue regeneration process. Finally, we conclude that the squalene-loaded emulgel scaffold could be an effective formulation used in the treatment of the burnt skin tissue defects.
全层烧伤因其自我修复能力受限，给临床医生带来了巨大的挑战。尽管已经有几种方法被用于修复这些烧伤皮肤组织缺陷，但所有方法的效果都不尽如人意。此外，近年来，皮肤组织工程技术作为一种改善皮肤组织再生和克服传统方法缺点的有前途的方法已经出现。尽管之前的文献报道了角鲨烯油的伤口愈合效果，但在本研究中，我们首次开发了一种负载角鲨烯的基于乳凝胶的支架，作为一种潜在的皮肤再生的新方法。这种负载角鲨烯的琼脂基乳凝胶支架是通过使用卵磷脂作为乳化剂的物理交联技术制备的。X 射线衍射、傅里叶变换红外光谱和场发射扫描电子显微镜等表征研究表明，所开发的乳凝胶支架具有非晶态性质、化学相互作用和交联能力。与琼脂凝胶和阴性对照组相比，负载角鲨烯的乳凝胶支架显示出更好的伤口收缩效果。就组织病理学和最近的免疫组织化学发现而言，显然有证据表明，负载角鲨烯的乳凝胶促进了更快的血管生成和巨噬细胞极化，以增强烧伤伤口愈合。此外，研究结果还表明，将角鲨烯油纳入配方可增强胶原蛋白沉积并加速烧伤皮肤组织的再生过程。最后，我们得出结论，负载角鲨烯的乳凝胶支架可能是一种有效的治疗烧伤皮肤组织缺陷的制剂。
