耳蜗中的脑源性神经营养因子——一项可重复性研究。
Brain-derived nerve growth factor in the cochlea - a reproducibility study.
机构信息
Department of Otolaryngology, University of Manitoba, Winnipeg, Manitoba, R3A 1R9, Canada.
出版信息
J Otolaryngol Head Neck Surg. 2020 Jun 5;49(1):37. doi: 10.1186/s40463-020-00432-7.
OBJECTIVE
Brain-derived nerve growth factor (BDNF) plays an important role in cochlear development so it is plausible that it could restore hearing loss if delivered directly into the cochlea. We wished to confirm our previous report that a single intracochlear injection of brain-derived nerve growth factor (BDNF) was beneficial for hearing in guinea pigs. We wished to assess the reproducibility of our results and assess possible improved methods with a view to developing a clinical treatment for sensorineural hearing loss.
METHODS
CDDP was used to create partial hearing loss in 25 guinea pigs. After 30 days the animals underwent ABR testing and unilateral BDNF injection through the round window in one ear and saline injection into the other ear. After allowing possible effects to stabilize, thirty days later, ABR threshold testing was repeated to assess change in threshold.
RESULTS
Final ABR thresholds were 60-70 dB and were about 11 dB better in the ears treated with BDNF.
CONCLUSION
Our original finding that Intracochlear BDNF can improve hearing in guinea pigs was confirmed, but the improvement demonstrated by the methods in this paper is too small for clinical application.
目的
脑源性神经营养因子（BDNF）在耳蜗发育中起重要作用，因此，如果将其直接递送至耳蜗内，它有可能恢复听力损失。我们希望证实我们之前的报告，即单次耳蜗内注射脑源性神经营养因子（BDNF）有益于豚鼠的听力。我们希望评估我们结果的可重复性，并评估可能改进的方法，以期开发治疗感音神经性听力损失的临床治疗方法。
方法
使用顺铂（CDDP）在 25 只豚鼠中造成部分听力损失。30 天后，对动物进行 ABR 测试，并通过圆窗将 BDNF 单侧注射到一只耳朵，将盐水注射到另一只耳朵。在允许可能的效果稳定后，三十天后，再次进行 ABR 阈值测试以评估阈值的变化。
结果
最终 ABR 阈值为 60-70dB，在接受 BDNF 治疗的耳朵中约改善了 11dB。
结论
我们最初的发现，即耳蜗内 BDNF 可以改善豚鼠的听力，得到了证实，但本文方法所显示的改善对于临床应用来说太小了。
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