Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China; Key Laboratory of Chemical Biology and Molecular Engineering Ministry, Shanxi University, Taiyuan 030006, China.
Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China; College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China; Key Laboratory of Chemical Biology and Molecular Engineering Ministry, Shanxi University, Taiyuan 030006, China.
Chin J Nat Med. 2020 Jun;18(6):460-471. doi: 10.1016/S1875-5364(20)30053-4.
The quality of Astragali Radix (AR) was closely related to the growth period. However, the current commodity grades of AR were only divided by diameter but not directly related to the growth period, which leads to the contradiction between the grade standard and the quality evaluation index. Therefore, solving this problem will be the key for the quality evaluation of AR. The present study established a potential quality evaluation approach for the absolute growth years' wild Astragali Radix (WAR) and transplanted Astragali Radix (TAR) based on the chemical components and anti-heart failure efficacy through adopting a bare-handed sections approach to rapidly identify the growth years of WAR. In this study, the absolute growth years of WAR were obtained by identifying the growth rings of 1-6 growth years root through the methods. The contents of flavonoids and saponins in 2-6 growth years' WAR were determined by HPLC-UV-ELSD. The contents of 12 chemical components and the anti-fatigue failure effects of WAR (4-year-old) and TAR were compared on rat models of heart failure induced by doxorubicin. Meanwhile, NMR-based untargeted metabolomics studies were performed to investigate the regulative effects of WAR and TAR. The result shows that the numbers of growth rings were consistent with the actual growth periods of AR. The HPLC-UV-ELSD determination indicated that the content of total flavonoids in WAR was significantly higher than that in TAR. Pharmacodynamics analysis revealed that the effects of WAR on cardiac function parameters (EF, FS and LVIDs), contents of serum CK and BNP were superior to those of TAR. 13 metabolites of heart were identified that had a higher rate of change in WAR group than TAR. Overall, a rapid identification method for the growth years of WAR was established, and the fact that WAR were significantly better than TAR in the heart failure rats was first proved in the paper. This study provided a scientific basis for establishing a novel commodity specification and grade of AR for clinical rational drug use.
黄芪的质量与生长年限密切相关。然而，目前黄芪的商品规格仅按直径划分，与生长年限没有直接关系，导致等级标准与质量评价指标之间存在矛盾。因此，解决这个问题将是黄芪质量评价的关键。本研究采用徒手切片法快速鉴定野生黄芪（WAR）和移栽黄芪（TAR）的生长年限，建立了基于化学成分和抗心力衰竭功效的绝对生长年限野生黄芪和移栽黄芪的潜在质量评价方法。在本研究中，通过该方法鉴定了 1-6 年生根的生长轮，获得了 WAR 的绝对生长年限。采用 HPLC-UV-ELSD 法测定了 2-6 年生 WAR 中黄酮类和皂苷类成分的含量。比较了 4 年生 WAR 和 TAR 对阿霉素诱导心力衰竭大鼠模型的 12 种化学成分含量和抗疲劳衰竭作用。同时，采用基于 NMR 的非靶向代谢组学研究方法研究了 WAR 和 TAR 的调节作用。结果表明，生长轮数与 AR 的实际生长周期一致。HPLC-UV-ELSD 测定结果表明，WAR 总黄酮含量明显高于 TAR。药效学分析表明，WAR 对心功能参数（EF、FS 和 LVIDs）、血清 CK 和 BNP 含量的作用优于 TAR。鉴定出 13 种心脏代谢物，WAR 组的变化率明显高于 TAR 组。综上所述，建立了一种快速鉴定 WAR 生长年限的方法，首次证明 WAR 在心力衰竭大鼠中的疗效明显优于 TAR。本研究为建立新型黄芪商品规格和等级、临床合理用药提供了科学依据。
