Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, Maharashtra, 400076, India.
Laboratory of Tumor Biology, Angiogenesis and Nanomedicine Research, National Center for Cell Science, Pune, 411008, India.
Commun Biol. 2020 Jun 5;3(1):284. doi: 10.1038/s42003-020-1016-z.
Developing a nanotheranostic agent with better image resolution and high accumulation into solid tumor microenvironment is a challenging task. Herein, we established a light mediated phototriggered strategy for enhanced tumor accumulation of nanohybrids. A multifunctional liposome based nanotheranostics loaded with gold nanoparticles (AuNPs) and emissive graphene quantum dots (GQDs) were engineered named as NFGL. Further, doxorubicin hydrochloride was encapsulated in NFGL to exhibit phototriggered chemotherapy and functionalized with folic acid targeting ligands. Encapsulated agents showed imaging bimodality for in vivo tumor diagnosis due to their high contrast and emissive nature. Targeted NFGL nanohybrids demonstrated near infrared light (NIR, 750 nm) mediated tumor reduction because of generated heat and Reactive Oxygen Species (ROS). Moreover, NFGL nanohybrids exhibited remarkable ROS scavenging ability as compared to GQDs loaded liposomes validated by antitumor study. Hence, this approach and engineered system could open new direction for targeted imaging and cancer therapy.
开发一种具有更好的图像分辨率和在实体瘤微环境中高积累的纳米诊疗剂是一项具有挑战性的任务。在此，我们建立了一种光介导的光触发策略，以增强纳米杂化物在肿瘤中的积累。一种基于多功能脂质体的纳米诊疗剂，负载金纳米粒子（AuNPs）和发荧光的石墨烯量子点（GQDs），被命名为 NFGL。此外，盐酸阿霉素被封装在 NFGL 中，以表现出光触发化疗，并通过叶酸靶向配体进行功能化。由于其高对比度和发荧光的特性，封装的试剂表现出用于体内肿瘤诊断的成像双模性。由于产生的热量和活性氧（ROS），靶向 NFGL 纳米杂化物显示出近红外光（NIR，750nm）介导的肿瘤减少。此外，与负载 GQDs 的脂质体相比，NFGL 纳米杂化物表现出显著的 ROS 清除能力，这通过抗肿瘤研究得到了验证。因此，这种方法和设计的系统可以为靶向成像和癌症治疗开辟新的方向。
