Siddiqi Muhammad Zubair, Yeon Jun Mo, Choi Hanna, Lee Jae Hag, Kim Sang Yong, Wee Ji-Hyang, Im Wan Taek
Department of Biotechnology, Hankyong National University, 327 Jungang-ro, Anseong-si, Gyeonggi-do, 17579, Republic of Korea.
AceEMzyme Co., Ltd, Academic Industry Cooperation, 327 Jungang-ro, Anseong-si, Gyeonggi-do, 17579, Republic of Korea.
Curr Microbiol. 2020 Sep;77(9):2002-2007. doi: 10.1007/s00284-020-02066-4. Epub 2020 Jun 5.
A Gram-reaction negative, aerobic, non-motile, light yellow colored, and rod-shaped bacterium (designated Gr-4) isolated from granules of a wastewater treatment plant, was characterized by a polyphasic approach to clarify its taxonomic position. Strain Gr-4 was observed to grew optimally at 30 ºC and at pH 7.0 on R2A medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Gr-4 belongs to the genus Luteimonas of the family Xanthomonadaceae and was most closely related to Luteimonas padinae CDR SL 15 (99.1%), Luteimonas terricola DSM 22344 (98.5%) and Luteimonas arsenica 26-35 (97.6). The genome comprises 2,917,404 bp with a G+C content of 70.5 mol%. The ANI value between strain Gr-4 and Luteimonas padinae CDR SL 15 was 87.3%. The DNA-DNA relatedness value between strain Gr-4 and Luteimonas padinae CDR SL 15, Luteimonas terricola DSM 22344 was 36.4 ± 1.3% and 14.2 ± 1.7%, respectively. The predominant quinone was Q-8. The major fatty acids were iso-C, iso-C and summed feature 9 (comprising iso-Cω9c and/or C 10-methyl) supported the affiliation of strain Gr-4 to the genus Luteimonas. Moreover, the physiological, biochemical results, and low level of ANI and DNA-DNA relatedness value allowed the phenotypic and genotypic differentiation of strains Gr-4 from other Luteimonas species with validly published names. The novel isolate therefore represents a novel species, for which the name Luteimonas granuli sp. nov. is proposed, with the type strain Gr-4 (=KACC 16614 = JCM 18203).
从一家污水处理厂的颗粒中分离出的一株革兰氏阴性、需氧、无运动性、浅黄色、杆状细菌（命名为Gr-4），采用多相分类法对其进行表征以明确其分类地位。观察到菌株Gr-4在R2A培养基上于30 ºC和pH 7.0条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，菌株Gr-4属于黄单胞菌科的金黄单胞菌属，与帕氏金黄单胞菌CDR SL 15（99.1%）、土壤金黄单胞菌DSM 22344（98.5%）和砷金黄单胞菌26-35（97.6%）关系最为密切。基因组由2,917,404 bp组成，G+C含量为70.5 mol%。菌株Gr-4与帕氏金黄单胞菌CDR SL 15之间的ANI值为87.3%。菌株Gr-与帕氏金黄单胞菌CDR SL 15、土壤金黄单胞菌DSM 22344之间的DNA-DNA杂交值分别为36.4 ± 1.3%和14.2 ± 1.7%。主要醌类为Q-8。主要脂肪酸为异-C、异-C和总和特征9（包括异-Cω9c和/或C 10-甲基），支持菌株Gr-4属于金黄单胞菌属。此外，生理生化结果以及低水平的ANI和DNA-DNA杂交值使得菌株Gr-4能够与其他已有效发表名称的金黄单胞菌属菌株在表型和基因型上进行区分。因此，该新分离株代表一个新物种，提议将其命名为颗粒金黄单胞菌（Luteimonas granuli sp. nov.），模式菌株为Gr-4（=KACC 16614 = JCM 18203）。
