Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstrasse 400, 01328, Dresden, Germany.
Environ Sci Pollut Res Int. 2020 Sep;27(25):32048-32061. doi: 10.1007/s11356-020-09525-2. Epub 2020 Jun 6.
Experiments conducted over a period of 6 weeks using Brassica napus callus cells grown in vitro under Eu(III) or U(VI) stress showed that B. napus cells were able to bioassociate both potentially toxic metals (PTM), 628 nmol Eu/g and 995 nmol U/g. Most of the Eu(III) and U(VI) was found to be enriched in the cell wall fraction. Under high metal stress (200 μM), cells responded with reduced cell viability and growth. Subsequent speciation analyses using both metals as luminescence probes confirmed that B. napus callus cells provided multiple-binding environments for Eu(III) and U(VI). Moreover, two different inner-sphere Eu species could be distinguished. For U(VI), a dominant binding by organic and/or inorganic phosphate groups of the plant biomass can be concluded.
使用 Brassica napus 愈伤组织细胞进行的为期 6 周的实验表明，在 Eu(III)或 U(VI)胁迫下体外生长的细胞能够生物结合这两种潜在毒性金属（PTM），Eu 的含量为 628 nmol/g，U 的含量为 995 nmol/g。大部分 Eu(III)和 U(VI)富集在细胞壁部分。在高金属胁迫（200 μM）下，细胞的活力和生长受到抑制。随后使用这两种金属作为荧光探针进行的形态分析证实，B. napus 愈伤组织细胞为 Eu(III)和 U(VI)提供了多种结合环境。此外，可以区分两种不同的内球 Eu 物种。对于 U(VI)，可以得出结论，植物生物质中的有机和/或无机磷酸盐基团占据主导地位。
