Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstraße 400, 01328 Dresden, Germany.
HZDR Innovation GmbH, Bautzner Landstraße 400, 01328 Dresden, Germany; VKTA - Strahlenschutz, Analytik & Entsorgung Rossendorf e.V., Bautzner Landstraße 400, 01328 Dresden, Germany.
Ecotoxicol Environ Saf. 2023 Apr 1;254:114741. doi: 10.1016/j.ecoenv.2023.114741. Epub 2023 Mar 9.
For the reliable safety assessment of repositories of highly radioactive waste, further development of the modelling of radionuclide migration and transfer in the environment is necessary, which requires a deeper process understanding at the molecular level. Eu(III) is a non-radioactive analogue for trivalent actinides, which contribute heavily to radiotoxicity in a repository. For in-depth study of the interaction of plants with trivalent f elements, we investigated the uptake, speciation, and localization of Eu(III) in Brassica napus plants at two concentrations, 30 and 200 µM, as a function of the incubation time up to 72 h. Eu(III) was used as luminescence probe for combined microscopy and chemical speciation analyses of it in Brassica napus plants. The localization of bioassociated Eu(III) in plant parts was explored by spatially resolved chemical microscopy. Three Eu(III) species were identified in the root tissue. Moreover, different luminescence spectroscopic techniques were applied for an improved Eu(III) species determination in solution. In addition, transmission electron microscopy combined with energy-dispersive X-ray spectroscopy was used to localize Eu(III) in the plant tissue, showing Eu-containing aggregates. By using this multi-method setup, a profound knowledge on the behavior of Eu(III) within plants and changes in its speciation could be obtained, showing that different Eu(III) species occur simultaneously within the root tissue and in solution.
为了可靠地评估高放射性废物库的安全性，有必要进一步开发环境中放射性核素迁移和转化的建模，这需要在分子水平上有更深的过程理解。Eu(III)是三价锕系元素的非放射性类似物，在废物库中对辐射毒性有很大贡献。为了深入研究植物与三价 f 元素的相互作用，我们研究了 Eu(III)在油菜植株中的摄取、形态和定位，浓度分别为 30 和 200 μM，作为随孵育时间至 72 小时的函数。Eu(III)被用作共焦显微镜和化学形态分析的荧光探针，用于研究油菜植物中的 Eu(III)。通过空间分辨化学显微镜探索了生物相关 Eu(III)在植物部分的定位。在根组织中鉴定出三种 Eu(III)物种。此外，应用不同的发光光谱技术来改进溶液中 Eu(III)物种的确定。此外，还使用透射电子显微镜结合能量色散 X 射线光谱法在植物组织中定位 Eu(III)，显示出含 Eu 的聚集体。通过使用这种多方法设置，可以深入了解 Eu(III)在植物体内的行为及其形态变化，表明不同的 Eu(III)物种同时存在于根组织和溶液中。
