Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstraße 400, 01328 Dresden, Germany.
Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstraße 400, 01328 Dresden, Germany.
J Hazard Mater. 2021 Jun 15;412:125251. doi: 10.1016/j.jhazmat.2021.125251. Epub 2021 Jan 28.
We have investigated the interaction of the actinide Cm(III) and its lanthanide homologue Eu(III) with cells of Brassica napus in suspension. This study combines biochemical techniques (plant cell response) with spectroscopic experiments to determine the chemical speciation of hazardous metals in contact with the plant cells. Experiments conducted over a period of 7 d showed that B. napus cells were able to bioassociate both potentially toxic metals in significant amounts up to 0.58 µmol Eu/g and 1.82 µmol Cm/g at 30 µM Eu(III) and 0.68 µM Cm(III), respectively. For Cm(III), a biosorption process could be identified as soon as 5 h post-exposure with 73 ± 4% of the Cm(III) bioassociated. Luminescence spectroscopy results based on UV and site-selective excitation confirmed the existence of three Cm(III)/Eu(III) [M(III)] species in both the supernatants and cells. The findings detailed herein support that M(III) coordinates to two kinds of carboxyl groups and phosphate groups.
我们研究了锕系元素 Cm(III)及其镧系同系物 Eu(III)与悬浮培养的油菜细胞的相互作用。本研究将生化技术（植物细胞反应）与光谱实验相结合，以确定与植物细胞接触的有害金属的化学形态。在 7 天的实验中表明，油菜细胞能够以显著的量生物结合这两种潜在的有毒金属，在 30 μM Eu(III)和 0.68 μM Cm(III)下，Eu/g 和 Cm/g 分别达到 0.58 μmol 和 1.82 μmol。对于 Cm(III)，在暴露后 5 小时即可识别出生物吸附过程，其中 73±4%的 Cm(III)被生物结合。基于紫外和位点选择性激发的荧光光谱结果证实了在上清液和细胞中存在三种 Cm(III)/Eu(III)[M(III)]物种。本文详述的研究结果表明，M(III)与两种类型的羧基和磷酸基团配位。
