Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Medical School, Federal University of Roraima, Boa Vista, RR, Brazil; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.
Toxicon. 2020 Sep;184:116-121. doi: 10.1016/j.toxicon.2020.05.027. Epub 2020 Jun 4.
Bushmasters (Lachesis spp) and lancehead vipers (Bothrops spp) are two of the most dangerous snakes found in Latin America. Victims of envenoming by these snakes require urgent administration of antivenom. Here, we report the identification of a small set of broadly neutralizing human monoclonal single-chain variable fragment (scFv) antibodies targeting key phospholipases A from Lachesis and Bothrops spp using phage display technology and demonstrate their in vitro efficacy using a hemolysis assay.
巨蝮属（Lachesis spp）和矛头蝮属（Bothrops spp）是拉丁美洲最危险的两种蛇类之一。被这些蛇咬伤的患者需要紧急使用抗蛇毒血清治疗。在这里，我们利用噬菌体展示技术，鉴定了一小部分针对巨蝮属和矛头蝮属中关键磷脂酶 A 的广谱中和人源单链可变片段（scFv）抗体，并通过溶血试验证明了它们的体外疗效。
