Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt; Nawah Scientific, Mokkatam, Cairo, Egypt.
Biomed Pharmacother. 2020 Aug;128:110302. doi: 10.1016/j.biopha.2020.110302. Epub 2020 Jun 5.
Breast cancer is one of the most commonly diagnosed cancer among women globally. Shogaol, the active constituent of many spices belonging to the Zingiberaceae family, has received wide attention among other shogaols in terms of its anticancer activity against different neoplasms. To date, its efficacy at the detailed molecular level against breast cancer has not been established.
In the current study, we investigated the cytotoxic potential and the underlying molecular details of 6-shogaol against breast adenocarcinomacells (MCF-7), and breast ductal carcinoma cells (T47D). Cytotoxicity assay, cell cycle analysis. Real-time PCR (qPCR), apoptosis and autophagy techniques were used for the determination and molecular investigation of its anticancer properties.
The current study shows that, Notch signaling downregulation (Hes1 and CyclinD1 genes), caused by 6-shogaol, lead to antiproliferative activity in breast cancer cells. The study further shows that treatment with 6-shogaol induced significant and time dependent cell cycle accumulation in G/M-phase. 6-Shogaol also induced significant apoptosis in breast cancer cells. Interestingly, 6-shogaol inhibited autophagy in breast cancer cell lines, which might force these cells to undergo apoptosis.
6-Shogaol is a promising candidate to be considered as a treatment of breast cancer.
乳腺癌是全球女性最常见的癌症之一。姜烯酚是姜科多种香料的活性成分，其在针对不同肿瘤的抗癌活性方面受到了广泛关注。迄今为止，其在乳腺癌方面的详细分子水平疗效尚未确定。
在本研究中，我们研究了 6-姜烯酚对乳腺腺癌细胞（MCF-7）和乳腺导管癌细胞（T47D）的细胞毒性潜力及其潜在的分子细节。使用细胞毒性测定、细胞周期分析、实时 PCR（qPCR）、凋亡和自噬技术来确定和研究其抗癌特性。
本研究表明，6-姜烯酚引起的Notch 信号下调（Hes1 和 CyclinD1 基因）导致乳腺癌细胞的抗增殖活性。研究进一步表明，6-姜烯酚处理导致 G/M 期细胞周期明显且时间依赖性积累。6-姜烯酚还诱导乳腺癌细胞发生显著的凋亡。有趣的是，6-姜烯酚抑制了乳腺癌细胞系中的自噬，这可能迫使这些细胞发生凋亡。
6-姜烯酚是一种很有前途的候选药物，可以考虑用于治疗乳腺癌。
