Monwar S, Begum H, Boby F
Dr Shabiha Monwar, Associate Professor of Microbiology, Marks Medical College, Dhaka, Bangladesh; E-mail:
Mymensingh Med J. 2020 Apr;29(2):420-423.
Dermatophytosis is an infection with fungi related to the skin, hair and/or nails. Fungal infections' treatment orally in dermatology has become a superior modality for the management of these conditions. In spite of increasing the number of antifungal drugs, treatment failure has been noticed against some cases as well as some relapse cases. To eradicate dermatophyte it is necessary to perform in vitro antifungal susceptibility testing of dermatophyte. Simple disk diffusion method in vitro assay may be used to assess antifungal propensity in dermatophytes. This cross sectional study was aimed to evaluate the antifungal activity of four antifungal drugs against several fresh clinical dermatophyte isolates by disk diffusion method at the out patient department with the collaboration of Microbiology department, Mymensingh Medical College, Mymensingh, Bangladesh from July 2013 to September 2014. Fifty three clinical dermatophytes species were isolated from patients suspected of having active dermatophytosis. Terbinafine, griseofulvin, fluconazole and itraconazole containing paper disks were used in disk diffusion method for evaluation in vitro activity of antifungal agents by measuring the mean diameter of inhibition around the disks. The isolates belong to three genera and three species as: Trichophyton Rubrum 44(83.04%), T. mentagrophytes 5(9.43%), Epidermophyton floccosum 4(7.55%). All isolates were significant to terbinafine. Terbinafine, itraconazole and griseofulvin were exposed in this study as the most ideal antifungal drugs for the treatment of dermatophytosis. For the detection of antifungal susceptibility of dermatophytes disk diffusion method is not only a simple but also a valuable method.
皮肤癣菌病是一种由与皮肤、毛发和/或指甲相关的真菌引起的感染。在皮肤病学中，真菌感染的口服治疗已成为管理这些病症的一种优越方式。尽管抗真菌药物的数量在增加，但仍注意到一些病例治疗失败以及一些复发情况。为了根除皮肤癣菌，有必要对皮肤癣菌进行体外抗真菌药敏试验。体外简单的纸片扩散法可用于评估皮肤癣菌的抗真菌倾向。本横断面研究旨在通过纸片扩散法，于2013年7月至2014年9月在孟加拉国迈门辛医学院微生物学系的协作下，在门诊部评估四种抗真菌药物对几种新鲜临床皮肤癣菌分离株的抗真菌活性。从疑似患有活动性皮肤癣菌病的患者中分离出53种临床皮肤癣菌。在纸片扩散法中使用含特比萘芬、灰黄霉素、氟康唑和伊曲康唑的纸片，通过测量纸片周围抑菌圈的平均直径来评估抗真菌剂的体外活性。分离株属于三个属和三个种，即：红色毛癣菌44株（83.04%）、须癣毛癣菌5株（9.43%）、絮状表皮癣菌4株（7.55%）。所有分离株对特比萘芬均敏感。在本研究中，特比萘芬、伊曲康唑和灰黄霉素被证明是治疗皮肤癣菌病最理想的抗真菌药物。对于检测皮肤癣菌的抗真菌药敏，纸片扩散法不仅是一种简单而且有价值的方法。
