绿色合成银纳米颗粒与传统抗真菌药物对指间毛癣菌、红色毛癣菌和絮状表皮癣菌的体外抗真菌活性比较
In Vitro Antifungal Activity of Green Synthesized Silver Nanoparticles in Comparison to Conventional Antifungal Drugs Against Trichophyton Interdigitale, Trichophyton Rubrum and Epidermophyton Floccosum.
作者信息
Mahmoudi Shahram, Vahidi Mahmoud, Malekabad Ebadollah Shiri, Izadi Alireza, Khatami Mehrdad, Dadashi Alireza
机构信息
Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Aja University of Medical Sciences, Tehran, Iran.
Department of Social Medicine, School of Medicine, Aja University of Medical Sciences, Tehran, Iran.
出版信息
Infect Disord Drug Targets. 2021;21(3):370-374. doi: 10.2174/1871526520666200715095744.
BACKGROUND
Dermatophytosis is a globally distributed fungal infection. Treatment failure and relapse is common in this disease. Silver nanoparticles are known for their promising antimicrobial activity. The aim of this study was to determine the antifungal activity of these nanoparticles against common dermatophyte species.
METHODS
A set of 30 molecularly identified dermatophytes including Trichophyton interdigitale (n=10), Trichophyton rubrum (n=10), and Epidermophyton floccosum (n=10) were used in this study. Green synthesized silver nanoparticles using chicory (Cichorium intybus) were tested for their antifungal activity in comparison to fluconazole, itraconazole and terbinafine. Interspecies differences in minimum inhibitory concentrations of antifungal drugs and silver nanoparticles were tested using Kruskal-Wallis test in SPSS software version 21.
RESULTS
The highest minimum inhibitory concentrations (MICs) among antifungal drugs were observed for fluconazole [range: 4-64 μg/mL, geometric mean (GM) =17.959 μg/mL], followed by itraconazole (range: 0.008-0.5, GM= 0.066) and terbinafine (range: 0.004-0.25 μg/mL, GM=0.027 μg/mL). Silver nanoparticles showed potent antifungal activity against all dermatophyte isolates with MICs (range: 0.25-32 μg/mL, GM=4.812 μg/mL) higher than those of itraconazole and terbinafine, but lower than fluconazole. MIC values of silver nanoparticles demonstrated significant differences between species (P=0.044), with E. floccosum having the highest MICs (GM=9.849 μg/mL) compared to T. interdigitale (GM=3.732 μg/mL) and T. rubrum (GM=3.031 μg/mL).
CONCLUSION
Silver nanoparticles demonstrated promising anti-dermatophyte activity against the studied dermatophytes. Due to their wide-spectrum activity against other fungal and bacterial pathogens, they could be a potential choice, at least in the case of cutaneous and superficial infections.
背景
皮肤癣菌病是一种全球分布的真菌感染。该病治疗失败和复发很常见。银纳米颗粒以其有前景的抗菌活性而闻名。本研究的目的是确定这些纳米颗粒对常见皮肤癣菌种类的抗真菌活性。
方法
本研究使用了一组30株经分子鉴定的皮肤癣菌，包括指间毛癣菌（n = 10）、红色毛癣菌（n = 10）和絮状表皮癣菌（n = 10）。将使用菊苣（菊苣）绿色合成的银纳米颗粒与氟康唑、伊曲康唑和特比萘芬相比，测试其抗真菌活性。使用SPSS 21软件中的Kruskal-Wallis检验来测试抗真菌药物和银纳米颗粒最低抑菌浓度的种间差异。
结果
抗真菌药物中观察到最高最低抑菌浓度（MIC）的是氟康唑[范围：4 - 64μg/mL，几何平均数（GM）= 17.959μg/mL]，其次是伊曲康唑（范围：0.008 - 0.5，GM = 0.066）和特比萘芬（范围：0.004 - 0.25μg/mL，GM = 0.027μg/mL）。银纳米颗粒对所有皮肤癣菌分离株均显示出强效抗真菌活性，其MIC（范围：0.25 - 32μg/mL，GM = 4.812μg/mL）高于伊曲康唑和特比萘芬，但低于氟康唑。银纳米颗粒的MIC值在不同菌种之间存在显著差异（P = 0.044），与指间毛癣菌（GM = 3.732μg/mL）和红色毛癣菌（GM = 3.031μg/mL）相比，絮状表皮癣菌的MIC最高（GM = 9.849μg/mL）。
结论
银纳米颗粒对所研究的皮肤癣菌显示出有前景的抗皮肤癣菌活性。由于它们对其他真菌和细菌病原体具有广谱活性，至少在皮肤和浅表感染的情况下，它们可能是一个潜在的选择。
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