Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), No 2 Yuanmingyuan West Rd, Haidian, Beijing, 100193, China.
Department of Livestock Services of Ministry of Fisheries and Livestock, Farmgate, Dhaka, 1215, Bangladesh.
Genes Genomics. 2020 Aug;42(8):893-899. doi: 10.1007/s13258-020-00937-5. Epub 2020 Jun 6.
Litter size is the most important reproductive trait which plays a crucial role in goat production. Therefore, improvement of litter size trait has been of increasing interest in goat industry as small improvement in litter size may lead to large profit. The recent Cashmere goat breeding program produced a high-reproductive genetic line of Arbas Cashmere goat. But the genetic mechanism of high reproduction rate remains largely unknown in this Chinese native goat breed. To address this question, we performed a genome-wide association studies (GWAS) using two groups of goats varying in fecundity.
Our study was aimed to investigate the significant SNPs and genes associated with high reproduction trait in Inner Mongolia Arbas Cashmere Goat.
We used logistic model association to perform GWAS using 47 goats from high fecundity group (~ 190%) and 314 goats from low fecundity group (~ 130%) of the Arbas Cashmere goat breed.
We identified 66 genomic regions associated with genome wide significant level wherein six loci were found to be associated with reproduction traits. Further analysis showed that five key candidate genes including KISS1, KHDRBS2, WNT10B, SETDB2 and PPP3CA genes are involved in goat fecundity trait. Gene ontology enrichment analysis revealed that several biological pathways could be involved in the variation of fecundity in female goats.
The identified significant SNPs or genes provide useful information about the underlying genetic control of fecundity trait which will be helpful to use them in goat breeding programs for improving the reproductive efficiency of goats.
产仔数是最重要的繁殖性状，对山羊生产起着至关重要的作用。因此，提高产仔数性状一直是山羊业日益关注的问题，因为产仔数的微小提高可能带来巨大的利润。最近的绒山羊育种计划培育出了高产绒的阿巴斯绒山羊高繁殖力遗传品系。但在这种中国本土山羊品种中，高繁殖率的遗传机制仍知之甚少。为了解决这个问题，我们使用两组产仔数不同的山羊进行了全基因组关联研究（GWAS）。
本研究旨在探讨内蒙古阿巴斯绒山羊高繁殖性状相关的显著 SNP 和基因。
我们使用逻辑模型关联，对高产羔组（190%）的 47 只山羊和低产羔组（130%）的 314 只山羊进行了 GWAS。
我们确定了 66 个与全基因组显著水平相关的基因组区域，其中 6 个位点与繁殖性状相关。进一步分析表明，包括 KISS1、KHDRBS2、WNT10B、SETDB2 和 PPP3CA 在内的 5 个关键候选基因参与了山羊产仔数性状。基因本体富集分析表明，几个生物学途径可能参与了雌性山羊产仔数的变异。
鉴定的显著 SNP 或基因提供了有关产仔数性状遗传控制的有用信息，这将有助于在山羊育种计划中利用这些信息来提高山羊的繁殖效率。
