National Agriculture and Food Research Center (NARO), Kyushu Okinawa Agricultural Research Center, Koshi, Kumamoto, Japan.
NARO, Central Region Agricultural Research Center, Tsukuba, Ibaraki, Japan.
Pest Manag Sci. 2020 Dec;76(12):3945-3953. doi: 10.1002/ps.5942. Epub 2020 Jul 17.
Root-knot nematodes (RKN, Meloidogyne spp.) are harmful phytophagous pests of Solanum spp. Some RKN species are becoming worldwide problems because of their virulence to RKN-resistant Solanum species. A new Solanum species carrying broad-spectrum resistance to Meloidogyne spp. is required for the effective management of this pest. Here we sought to determine the host suitability of RKN to Solanum palinacanthum, a wild Solanum species, and to evaluate its potential effectiveness in RKN management.
We identified an RKN-resistant Solanum species, S. palinacanthum, by screening Solanum accessions. We tested its spectrum of resistance to common Meloidogyne spp. in Japan. In pot tests inoculated with second-stage juveniles, S. palinacanthum showed poor host suitability for Melidogyne incognita, M. arenaria genotypes A2-J and A2-O, M. javanica and M. hapla, indicating broad-spectrum resistance to RKN. The development of M. incognita within S. palinacanthum roots was significantly poorer than that in susceptible S. melongena and S. lycopersicum at 10 and 21 days after inoculation. Microplot tests confirmed that the number of second-stage juveniles in plots where S. palinacanthum grew and root galling of the root system were significantly lower than those of susceptible S. melongena, suggesting that the resistance could be used to manage RKN under field conditions.
S. palinacanthum showed poor host suitability to all Meloidogyne spp. tested in this study, and it thus has the potential to be used as a genetic resource with broad-spectrum RKN resistance, and it could be effective against multiple RKN species in a field. © 2020 Society of Chemical Industry.
根结线虫（RKN，Meloidogyne spp.）是茄属植物的有害植食性害虫。由于某些 RKN 物种对 RKN 抗性茄属物种的毒力，它们正在成为全球性问题。需要一种新的广谱抗 Meloidogyne spp. 的茄属植物来有效管理这种害虫。在这里，我们试图确定 RKN 对野生茄属植物 S. palinacanthum 的宿主适宜性，并评估其在 RKN 管理中的潜在有效性。
我们通过筛选茄属植物品系鉴定了一种 RKN 抗性茄属植物 S. palinacanthum。我们在日本测试了其对常见 Meloidogyne spp. 的抗性谱。在接种二龄幼虫的盆栽试验中，S. palinacanthum 对 M. incognita、M. arenaria 基因型 A2-J 和 A2-O、M. javanica 和 M. hapla 的宿主适宜性较差，表明对 RKN 具有广谱抗性。在接种后 10 和 21 天，M. incognita 在 S. palinacanthum 根内的发育明显比易感的 S. melongena 和 S. lycopersicum 差。小区试验证实，S. palinacanthum 生长的小区中二龄幼虫数量和根系根瘤的数量明显低于易感的 S. melongena，表明该抗性可用于田间管理 RKN。
S. palinacanthum 对本研究中测试的所有 Meloidogyne spp. 的宿主适宜性均较差，因此具有作为广谱 RKN 抗性遗传资源的潜力，并且在田间可能对多种 RKN 物种有效。 © 2020 化学工业协会。
