Anto Suganya Regi, Jeya Jothi Gabriel
Department of Plant Biology and Biotechnology, Loyola College, Nungambakkam, Chennai-600034, Tamil Nadu, India.
J Tradit Chin Med. 2020 Jun;40(3):473-483. doi: 10.19852/j.cnki.jtcm.2020.03.017.
To explore the total phenolic and flavonoid content, enzymatic, non-enzymatic antioxidant properties, anti-inflammation and anticancer activities of hexane, ethyl acetate and methanol extracts of Floscopa scandens (F. scandens).
Non-enzymatic antioxidant activity was examined by 2, 2-diphenyl-1-picrylhydrazyl assay, nitric oxide scavenging assay, hydroxyl radical scavenging assay, reducing power assay, hydrogen peroxide scavenging assay, superoxide scavenging assay and metal chelating assay. Enzymatic antioxidant ability was screened for the antioxidant enzymes such as ascorbate oxidase, peroxidase, catalase and polyphenol oxidase. The anti-inflammatory property was proved with the inhibition of protein denaturation and protease inhibitory assays. In vitro anticancer activity was assessed by cell viability assay.
Methanol extract contained high amount of phenols (198.41 mg catechol equivalent/gram extract) and flavonoids (101.70 mg quercetin equivalent/gram extract) showed higher activity than hexane and ethyl acetate extracts in all experiments. Fresh plant showed considerable enzymatic antioxidant activity.
The results revealed that the methanol extracts of F. scandens could be used as a potential source of antioxidant, anti-inflammatory and anticancer bioactive compounds.
探讨节节菜的正己烷、乙酸乙酯和甲醇提取物中的总酚和黄酮含量、酶促和非酶促抗氧化特性、抗炎和抗癌活性。
通过2,2-二苯基-1-苦基肼测定法、一氧化氮清除测定法、羟自由基清除测定法、还原能力测定法、过氧化氢清除测定法、超氧阴离子清除测定法和金属螯合测定法检测非酶促抗氧化活性。筛选抗坏血酸氧化酶、过氧化物酶、过氧化氢酶和多酚氧化酶等抗氧化酶的酶促抗氧化能力。通过抑制蛋白质变性和蛋白酶抑制试验证明其抗炎特性。通过细胞活力试验评估体外抗癌活性。
甲醇提取物含有大量酚类（198.41毫克儿茶酚当量/克提取物）和黄酮类（101.70毫克槲皮素当量/克提取物），在所有实验中均显示出比正己烷和乙酸乙酯提取物更高的活性。新鲜植物显示出相当的酶促抗氧化活性。
结果表明，节节菜的甲醇提取物可作为抗氧化、抗炎和抗癌生物活性化合物的潜在来源。
