Department of Allergy, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Beijing Key Laboratory of Precision Medicine for Diagnosis and Treatment on Allergic Diseases, Beijing, China.
Immunol Invest. 2021 May;50(4):389-398. doi: 10.1080/08820139.2020.1770783. Epub 2020 Jun 8.
: The cross-reactive allergen between mugwort () and kidney bean () has not yet been identified.: A total of 24 patients were included in this study. The sera of patients were analyzed for the concentrations of specific IgE antibodies. The allergenicity and cross-reactivity were investigated by Western blotting and immunoblot inhibitory experiments.: The immunoblotting indicated the binding of patients' IgE to crude mugwort extract at ~26 kDa protein (15 cases), ~60 kDa (15 cases), and 10-15 kDa proteins (12 cases). The results of the immunoblot-inhibition assay showed that kidney bean seed extract inhibited specific IgE binding to mugwort at 10-15 kDa, ~26 kDa, and ~60 kDa in 4 (16.7%), 1 (4.2%) and 2 (8.3%) cases, respectively. On the other hand, mugwort extract was demonstrated to inhibit specific IgE binding to kidney bean seed at 10-15 kDa, 15-20 kDa, ~30 kDa, and 60 kDa in 1 (4.2%), 3 (12.5%), 4 (16.7%), and 3 (12.5%) cases, respectively.: The 26-30 kDa, 10-15 kDa, and 60 kDa proteins are potential causative agents of the cross-reactivity between mugwort and kidney beans. The findings of this study improved the current understanding on the allergenicity of kidney beans and would provide insights into the refinement of treatment strategy for anaphylaxis.
: 艾蒿（）和菜豆（）之间的交叉过敏原尚未确定。: 本研究共纳入 24 例患者。分析患者血清中特异性 IgE 抗体浓度。通过 Western 印迹和免疫印迹抑制实验研究过敏原性和交叉反应性。: 免疫印迹表明，患者 IgE 与粗艾蒿提取物在约 26 kDa 蛋白（15 例）、约 60 kDa（15 例）和 10-15 kDa 蛋白（12 例）处结合。免疫印迹抑制试验结果显示，菜豆种子提取物分别在 4 例（16.7%）、1 例（4.2%）和 2 例（8.3%）中抑制了特异性 IgE 与艾蒿在 10-15 kDa、26 kDa 和60 kDa 处的结合。另一方面，艾蒿提取物被证明在 1 例（4.2%）、3 例（12.5%）、4 例（16.7%）和 3 例（12.5%）中分别抑制了特异性 IgE 与菜豆种子在 10-15 kDa、15-20 kDa、~30 kDa 和 60 kDa 处的结合。: 26-30 kDa、10-15 kDa 和 60 kDa 蛋白是艾蒿和菜豆之间交叉反应的潜在原因。本研究的发现提高了对菜豆过敏原性的认识，并为过敏反应治疗策略的完善提供了思路。
