College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China.
Carbohydr Polym. 2020 Aug 1;241:116373. doi: 10.1016/j.carbpol.2020.116373. Epub 2020 Apr 29.
Developing green and smart emulsifier for multiple responsive Pickering emulsion has been a great significance to many industries. Therefore, a novel emulsifier, poly (N-isopropylacrylamide) (PNIPAM) grafted chitosan (CS) microgel (CS-g-PNIPAM), was obtained, which exhibited swelling and deswelling behaviors in response to abrupt changes of pH and temperature. The resulting O/W Pickering emulsion was also sensitive to pH and temperature, and adjusting either the pH from 5.0 to 9.0 or temperature from 25 to 45 ℃ could effectively trigger oil-water separation and emulsification on demand. Based on it, a biphasic biocatalysis was applied to evaluate the catalytic capacity of this approach. The hydrolysis of fish oil and esterification of oleic acid with n-octanol were performed successfully. Meanwhile, the easy separation of products and good recyclability could be realized by reversibly increasing or decreasing temperature. The green system provides a great potential for the application of biopolymers in biphasic interfacial biocatalysis.
开发用于多种响应性 Pickering 乳液的绿色智能乳化剂对许多行业具有重要意义。因此，获得了一种新型乳化剂，即聚（N-异丙基丙烯酰胺）（PNIPAM）接枝壳聚糖（CS）微凝胶（CS-g-PNIPAM），其在 pH 和温度的急剧变化下表现出溶胀和收缩行为。所得的 O/W Pickering 乳液也对 pH 和温度敏感，调节 pH 值从 5.0 到 9.0 或温度从 25 到 45°C 可有效按需触发油水分离和乳化。在此基础上，采用两相生物催化法来评估该方法的催化能力。成功地进行了鱼油的水解和油酸与正辛醇的酯化。同时，通过可逆地升高或降低温度可以实现产物的易于分离和良好的可循环性。该绿色体系为生物聚合物在两相界面生物催化中的应用提供了巨大的潜力。
