Division of Rehabilitation Sciences, University of Texas Medical Branch, Galveston, TX, United States; Shriners Hospitals for Children, Galveston, TX, United States.
Department of Surgery, University of Texas Medical Branch, Galveston, TX, United States; Shriners Hospitals for Children, Galveston, TX, United States.
Burns. 2020 Nov;46(7):1653-1659. doi: 10.1016/j.burns.2020.04.034. Epub 2020 May 19.
Severe burns results in a prolonged hypermetabolic response. Brown adipose tissue (BAT), abundant in uncoupling protein 1 (UCP1), plays a key role in non-shivering thermogenesis. We set out to determine if BAT is recruited in response to severe burns.
Male balb-c mice underwent scald burns on approximately 20-25% of their total body surface. BAT was harvested from the interscapular fat pad of sham and burned mice at 3h, 24h, 4 days, and 10 days after injury. High-resolution respirometry was used to determine mitochondrial respiratory function in BAT. BAT protein concentration, and mitochondrial enzyme activity were also determined.
Respiration increased in BAT of burned mice, peaking at 24h after injury (after injury, P<0.001). While UCP1 independent respiration was not significantly altered by burn, UCP1 dependent respiration increased >2-fold at 24h after injury when compared to the 3h and sham group (P<0.01). Normalized to citrate synthase activity, total uncoupled (P<0.05) and UCP1 dependent (P<0.01) respiration remained elevated at 24h after injury.
We show a time-dependent recruitment of rodent BAT in response to severe burns. Given recent reports that humans, including patients with severe burns, have functional BAT, these data support a role for BAT in the hypermetabolic response to severe burns.
严重烧伤会导致长时间的代谢亢进反应。富含解偶联蛋白 1（UCP1）的棕色脂肪组织（BAT）在非颤抖性产热中起着关键作用。我们旨在确定 BAT 是否会因严重烧伤而被募集。
雄性 balb-c 小鼠的大约 20-25%的体表面积接受烫伤烧伤。在受伤后 3 小时、24 小时、4 天和 10 天，从假伤和烧伤小鼠的肩胛间脂肪垫中采集 BAT。使用高分辨率呼吸测定法来确定 BAT 中的线粒体呼吸功能。还测定了 BAT 蛋白浓度和线粒体酶活性。
烧伤小鼠的 BAT 呼吸增加，在受伤后 24 小时达到峰值（与受伤后相比，P<0.001）。虽然 UCP1 非依赖性呼吸未因烧伤而显著改变，但与 3 小时和假伤组相比，UCP1 依赖性呼吸在受伤后 24 小时增加了>2 倍（P<0.01）。与柠檬酸合酶活性归一化后，总解偶联（P<0.05）和 UCP1 依赖性（P<0.01）呼吸在受伤后 24 小时仍然升高。
我们显示了啮齿动物 BAT 对严重烧伤的时间依赖性募集。鉴于最近的报道表明人类（包括严重烧伤患者）具有功能性 BAT，这些数据支持 BAT 在严重烧伤的代谢亢进反应中的作用。
