School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, China.
State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China.
Eur J Mass Spectrom (Chichester). 2020 Aug;26(4):301-308. doi: 10.1177/1469066720926435. Epub 2020 Jun 6.
B. (NT), L. (HR), M. (LR), (LF), L. (RX), and L. f. (RC) are six berries from Tibetan Plateau. They have been used in traditional folk medicine with a long history, which are rich in anthocyanins. However, detailed study of their anthocyanins remains scarce. Therefore, a method for rapid simultaneous identification and quantification of 12 anthocyanins from berries using UPLC-Quadruple-Orbitrap MS system (UPLC-Q-Orbitrap MS) was established in this work. It was verified with limit of detection (3.86-11.61 µg/L), limit of quantification (3.86-11.61 µg/L), precision (0.95-2.38%), repeatability (0.96-2.08%), stability (0.86-2.31%), mean recovery (95.8-103.1%), recovery range (93.1-107.2%) and RSD less than 5.21%. It was then used in the analysis of anthocyanins in six berries species; 8, 7, 7, 7, 6 and 9 species of anthocyanins have been identified in NT, LF, LR, HR, RC and RX, respectively based on their own retention time and exact mass in positive mode, and for the first time quantified successfully in each berry (31.11 ± 0.42-2978 ± 25.67 μg.g). Finally, 2, 2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging activity (0.92 ± 0.12-5.61 ± 0.23 mM TE/100 g), ferric reducing antioxidant power (FRAP) (1.23 ± 0.15-7.42 ± 0.28 mM TE/100 g) and total antioxidant activity (T-AOC) assays were used to evaluate the antioxidant activities of the six berries.
B. (NT)、L. (HR)、M. (LR)、(LF)、L. (RX) 和 L. f. (RC) 是来自青藏高原的六种浆果。它们在传统民间医学中已有悠久的应用历史，富含花色苷。然而，对其花色苷的详细研究仍然很少。因此，本工作建立了一种使用 UPLC-Quadruple-Orbitrap MS 系统 (UPLC-Q-Orbitrap MS) 快速同时鉴定和定量分析来自浆果的 12 种花色苷的方法。通过检测限 (3.86-11.61 µg/L)、定量限 (3.86-11.61 µg/L)、精密度 (0.95-2.38%)、重复性 (0.96-2.08%)、稳定性 (0.86-2.31%)、平均回收率 (95.8-103.1%)、回收率范围 (93.1-107.2%) 和 RSD 小于 5.21% 进行了验证。然后将其用于分析六种浆果中花色苷的含量；根据其在正模式下的保留时间和精确质量，在 NT、LF、LR、HR、RC 和 RX 中分别鉴定出 8、7、7、7、6 和 9 种花色苷，并首次成功对每种浆果进行定量（31.11 ± 0.42-2978 ± 25.67 μg.g）。最后，采用 2,2-偶氮双(3-乙基苯并噻唑啉-6-磺酸) (ABTS) 自由基清除活性 (0.92 ± 0.12-5.61 ± 0.23 mM TE/100 g)、铁还原抗氧化能力 (FRAP) (1.23 ± 0.15-7.42 ± 0.28 mM TE/100 g) 和总抗氧化能力 (T-AOC) 测定法来评估六种浆果的抗氧化活性。
