School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China.
State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016, P. R. China.
Chem Biodivers. 2020 Apr;17(4):e2000033. doi: 10.1002/cbdv.202000033. Epub 2020 Mar 24.
Five traditional medicinal food from the Tibetan plateau including Nitraria tangutorum Bobrov (NT), Hippophae rhamnoides L. (HR), Lycium ruthenicum Murray (LR), Lycium barbarum L. (LB) and Rubus corchorifolius L.f. (RC) are rich in phenolic compounds. However, the detailed studies about the phenolic compounds remain scarce. Therefore, we established a rapid method for the simultaneous identification and quantification of the phenolic compounds from berries via Ultra Performance Liquid Chromatography-Quadruple-Orbitrap MS system (UPLC-Q-Orbitrap MS). This method was verified from many aspects including detection limit, quantification limit, precision, repeatability, stability, average recovery rate and recovery range, and then was used to analyze the phenolic compounds in these five species of berries. Finally, a total of 21 phenolic compounds were directly identified by comparing the retention time and exact mass, of which 14 compounds were identified by us for the first time in berries from the Tibetan plateau, including one flavonoid aglycone (myricetin), 11 phenolic acids (gallic acid, protocatechuate, chlorogenic acid, vanillic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, 2-hydroxybenzeneacetic acid and ellagic acid), one flavanol (catechin) and one dihydrochalcone flavonoid (phloretin). Quantitative results showed that rutin, myricetin, quercetin and kaempferol were the main flavonoids. Moreover, a variety of phenolic acid compounds were also detected in most of the berries from the Tibetan plateau. Among these compounds, the contents of protocatechuate and chlorogenic acid were high, and high levels of catechin and phloretin were also detected in these plateau berries.
来自青藏高原的五种传统药食同源植物，包括盐肤木（Nitraria tangutorum Bobrov）、沙棘（Hippophae rhamnoides L.）、黑果枸杞（Lycium ruthenicum Murray）、枸杞（Lycium barbarum L.）和蓬蘽（Rubus corchorifolius L.f.），均富含酚类化合物。然而，关于这些植物中酚类化合物的详细研究仍然很少。因此，我们建立了一种基于超高效液相色谱-四极杆轨道阱质谱联用系统（UPLC-Q-Orbitrap MS）的快速分析方法，用于同时鉴定和定量分析浆果中的酚类化合物。该方法从检测限、定量限、精密度、重复性、稳定性、平均回收率和回收率范围等方面进行了验证，然后用于分析这五种浆果中的酚类化合物。最后，通过比较保留时间和精确质量，共直接鉴定出 21 种酚类化合物，其中 14 种化合物是我们首次在青藏高原浆果中鉴定出的，包括一种类黄酮苷元（杨梅素）、11 种酚酸（没食子酸、原儿茶酸、绿原酸、香草酸、咖啡酸、丁香酸、对香豆酸、阿魏酸、2-羟基苯乙酸和鞣花酸）、一种黄烷醇（儿茶素）和一种二氢查耳酮类黄酮（根皮苷）。定量结果表明，芦丁、杨梅素、槲皮素和山柰酚是主要的类黄酮。此外，还检测到大多数来自青藏高原的浆果中含有多种酚酸化合物。在这些化合物中，原儿茶酸和绿原酸的含量较高，同时也检测到这些高原浆果中含有高水平的儿茶素和根皮苷。
