Hegazy Alaa H A, Galal Lamia A, Hassan Tasneem M, Khalifa Refaat M A
Asyut, Egypt.
J Parasit Dis. 2020 Jun;44(2):349-354. doi: 10.1007/s12639-020-01195-8. Epub 2020 Jan 24.
Schistosomiasis is a neglected tropical parasitic disease which has been controlled by praziquantel for many decades; however, reemergence of praziquantel resistant strains has been a continuous threat. Therefore, the development of reliable antischistosomal vaccine is significantly demanded for optimal control. In the present study, comparison among and crude antigens was carried out by Sodium dodecyl sulphate polyacrylamide gel electrophoresis. Hyperimmunization of rabbits with tested parasites' crude antigens was done to obtain hyperimmune sera. Western blotting was applied to show cross reactivity between parasites' crude antigens and either homologous or heterologous sera. Although there was no cross reaction between . crude antigens and sera of both species and vice versa; it is supposed that the immunogenic band at 79 kDa might develop cross reactivity with spp. SEA fractionation if used in future studies.
血吸虫病是一种被忽视的热带寄生虫病，几十年来一直通过吡喹酮进行控制；然而，耐吡喹酮菌株的再度出现一直是持续存在的威胁。因此，为了实现最佳控制，迫切需要开发可靠的抗血吸虫疫苗。在本研究中，通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳对[具体两种寄生虫名称未给出]和[具体两种寄生虫名称未给出]的粗抗原进行了比较。用受试寄生虫的粗抗原对兔子进行超免疫以获得超免疫血清。应用蛋白质印迹法来显示寄生虫粗抗原与同源或异源血清之间的交叉反应性。尽管[具体两种寄生虫名称未给出]的粗抗原与[具体两种寄生虫名称未给出]物种的血清之间没有交叉反应，反之亦然；但如果在未来研究中使用，推测79 kDa处的免疫原性条带可能与[具体血吸虫属物种名称未给出]的可溶性虫卵抗原（SEA）分级分离产生交叉反应。
