Bezerra-Santos Marcos Antônio, Fontes Carolina Silveira, Nogueira Bárbara Cristina Félix, Yamatogi Ricardo Seiti, Ramos Rafael Antonio Nascimento, Galhardo Juliana Arena, Furtado Luis Fernando Viana, Rabelo Élida Mara Leite, de Araújo Jackson Victor, Campos Artur Kanadani
Department of Veterinary Medicine, Federal University of Viçosa, Av. PH Rolfs, s/n, University Campus, Viçosa, MG 36570-900 Brazil.
Academic Unit of Garanhuns, Federal Rural University of Pernambuco, Av. Bom Pastor, s/n, Boa Vista, Garanhuns, PE 55292-270 Brazil.
J Parasit Dis. 2020 Jun;44(2):355-363. doi: 10.1007/s12639-020-01205-9. Epub 2020 Mar 2.
Currently, a great proportion of the emerging infectious human diseases are zoonotic, with most of the pathogens originated from wildlife. In this sense, synanthropic animals such as marsupials play important role in the dissemination of pathogens due to their proximity to human dwellings. These hosts are affected by many gastrointestinal parasites, including species with zoonotic potential. The aim of this study was to assess the diversity of gastrointestinal parasites infecting the black-eared opossum captured in urban areas of Southeastern, Brazil. In addition, the potential risk for the human population based on the One Health perspective has been discussed. Forty-nine marsupial specimens were captured with Tomahawk live traps and fecal samples were collected. The samples were evaluated by parasitological procedures. Eggs and oocysts were analyzed at different magnifications (400 × and 1000 ×), and their identification, together with adult nematodes, was established on morphological and morphometric data. Forty-three hosts (87.76%) scored positive for at least one gastrointestinal parasite, being 83.67% (41/49) for helminths, and 65.30% (32/49) for protozoa. For sp., only 13 samples were evaluated due to insufficient amount of feces obtained of some animals. A prevalence of 23.08% (3/13) was reported for this parasite. PCR analysis revealed Ancylostomatidae eggs to belong to the genus . Our results demonstrated that multiparasitism is frequently found in these animals and a high percentage of potentially zoonotic parasites are observed, implying that may be involved in zoonotic cycles in urban environments.
目前，很大一部分新出现的人类传染病是人畜共患病，大多数病原体源自野生动物。从这个意义上说，有袋类等伴人动物由于靠近人类住所，在病原体传播中发挥着重要作用。这些宿主受到许多胃肠道寄生虫的影响，包括具有人畜共患病潜力的物种。本研究的目的是评估感染巴西东南部城市地区捕获的黑耳负鼠的胃肠道寄生虫的多样性。此外，还从“同一健康”的角度讨论了对人类种群的潜在风险。用战斧式活套捕获了49个有袋类标本，并收集了粪便样本。通过寄生虫学程序对样本进行评估。在不同放大倍数（400×和1000×）下分析虫卵和卵囊，并根据形态学和形态测量数据对其以及成虫线虫进行鉴定。43只宿主（87.76%）至少感染一种胃肠道寄生虫呈阳性，其中感染蠕虫的占83.67%（41/49），感染原生动物的占65.30%（32/49）。对于某物种，由于部分动物获得的粪便量不足，仅对13个样本进行了评估。该寄生虫的感染率为23.08%（3/13）。PCR分析显示钩口科虫卵属于某属。我们的结果表明，这些动物中经常发现多重寄生虫感染，并且观察到高比例的潜在人畜共患寄生虫，这意味着有袋类可能参与城市环境中的人畜共患病传播循环。
