Bermúdez Sergio E, Gottdenker Nicole, Krishnvajhala Aparna, Fox Amy, Wilder Hannah K, González Kadir, Smith Diorene, López Marielena, Perea Milixa, Rigg Chystrie, Montilla Santiago, Calzada José E, Saldaña Azael, Caballero Carlos M, Lopez Job E
Departamento de Investigación en Entomología Médica, Instituto Conmemorativo Gorgas de Estudios de la Salud, Ciudad de Panamá, Panamá.
Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens, Georgia, United States of America.
PLoS One. 2017 Jan 6;12(1):e0169047. doi: 10.1371/journal.pone.0169047. eCollection 2017.
Synanthropic wild mammals can be important hosts for many vector-borne zoonotic pathogens. The aim of this study was determine the exposure of synanthropic mammals to two types of tick-borne pathogens in Panama, spotted fever group Rickettsia (SFGR) and Borrelia relapsing fever (RF) spirochetes. One hundred and thirty-one wild mammals were evaluated, including two gray foxes, two crab-eating foxes (from zoos), four coyotes, 62 opossum and 63 spiny rats captured close to rural towns. To evaluate exposure to SFGR, serum samples from the animals were tested by indirect immunofluorescence assay (IFA) using Rickettsia rickettsii and Candidatus Rickettsia amblyommii antigen. Immunoblotting was performed using Borrelia turicatae protein lysates and rGlpQ, to assess infection caused by RF spirochetes. One coyote (25%) and 27 (43%) opossums showed seroreactivity to SFGR. Of these opossums, 11 were seroreactive to C. R. amblyommii. Serological reactivity was not detected to B. turicatae in mammal samples. These findings may reflect a potential role of both mammals in the ecology of tick-borne pathogens in Panama.
伴人野生哺乳动物可能是许多媒介传播的人畜共患病原体的重要宿主。本研究的目的是确定巴拿马伴人哺乳动物对两种蜱传病原体的暴露情况，即斑点热群立克次体（SFGR）和回归热疏螺旋体（RF）。评估了131只野生哺乳动物，包括两只灰狐、两只食蟹狐（来自动物园）、四只郊狼、62只负鼠和63只在农村城镇附近捕获的刺豚鼠。为了评估对SFGR的暴露情况，使用立氏立克次体和嗜吞噬细胞无形体抗原通过间接免疫荧光试验（IFA）检测动物的血清样本。使用杜氏疏螺旋体蛋白裂解物和重组GlpQ进行免疫印迹，以评估由RF疏螺旋体引起的感染。一只郊狼（25%）和27只（43%）负鼠对SFGR呈血清反应阳性。在这些负鼠中，11只对嗜吞噬细胞无形体呈血清反应阳性。在哺乳动物样本中未检测到对杜氏疏螺旋体的血清学反应。这些发现可能反映了这两种哺乳动物在巴拿马蜱传病原体生态中的潜在作用。
