Bernardes Marta, Le Minh Duc, Nguyen Truong Quang, Pham Cuong The, Pham Anh Van, Nguyen Tao Thien, Rödder Dennis, Bonkowski Michael, Ziegler Thomas
Cologne Zoo, Riehler Str. 173, 50735 Cologne, Germany Cologne Zoo Cologne Germany.
Terrestrial Ecology, Institute of Zoology, University of Cologne, Zülpicher Str. 47b, 50674 Cologne, Germany University of Cologne Cologne Germany.
Zookeys. 2020 May 21;935:121-164. doi: 10.3897/zookeys.935.37138. eCollection 2020.
The complex from northern Vietnam is reviewed based on morphological comparisons and analysis of the mitochondrial marker NADH dehydrogenase subunit 2 (ND2). Based on molecular divergences, which were revealed to be higher than in other congeners, in concert with morphological differences, two new species and one subspecies are described herein: differs from by 3.2 to 3.6 % genetic divergence and a combination of distinct morphological characters, such as head slightly longer than wide, distinct mid-dorsal ridge, relatively wide distance between the eyes, tips of fingers reaching the eye when foreleg is laid forward, labial and gular folds present, central belly skin with tubercles shaped like transverse wrinkles and distinct, pointy to round rib nodules. The population of consists of two subclades, the nominotypic one occurring on the eastern side of the Da River (or Black River, including Hoa Binh and Phu Tho provinces), and another occurring on the western side (including Son La and Thanh Hoa provinces). These two subclades differ by 2.5 to 3.1 % genetic divergence and distinct morphological characters. The western subclade is herein described as , which differs from the nominotypic form by a wider head, longer and narrower snout, shorter femur length, and an overall less granulose skin, without an increased concentration of warts on the body sides. A second new species, is described from Lai Chau Province. It differs from by 4.1 to 4.2 % and from by 3.6 to 4.5 % genetic divergences as well as by a combination of distinct morphological characters, such as head longer than wide, tips of fingers reaching nostril when foreleg adpressed along head, rib nodules distinct, round and relatively enlarged, and wide distance between the eyes.
基于形态学比较和线粒体标记物烟酰胺腺嘌呤二核苷酸脱氢酶亚基2（ND2）的分析，对来自越南北部的复合体进行了综述。基于分子差异（结果显示其高于其他同属物种），结合形态学差异，本文描述了两个新物种和一个亚种：[物种名1]与[物种名2]的遗传差异为3.2%至3.6%，并具有一系列独特的形态特征组合，如头部略长于宽、明显的中背脊、两眼之间距离相对较宽、前肢向前伸展时手指尖可达眼部、有唇褶和喉褶、腹部中央皮肤有呈横向皱纹状的瘤以及明显的、尖形至圆形的肋骨结节。[物种名3]的种群由两个亚分支组成，指名亚种分布在大河（或黑河，包括和平省和富寿省）东侧，另一个分布在西侧（包括山罗省和清化省）。这两个亚分支的遗传差异为2.5%至3.1%，且具有明显的形态特征差异。本文将西侧亚分支描述为[新亚种名]，它与指名亚种的区别在于头部更宽、吻部更长且更窄、股骨长度更短以及整体皮肤颗粒状更少，身体两侧疣的浓度没有增加。第二个新物种[新物种名]来自莱州省。它与[物种名1]的遗传差异为4.1%至4.2%，与[物种名2]的遗传差异为3.6%至4.5%，还具有一系列独特的形态特征组合，如头部长于宽、前肢沿头部贴紧时手指尖可达鼻孔、肋骨结节明显、圆形且相对较大以及两眼之间距离较宽。
