Babcock University, Ilishan, Nigeria.
Department of Biological Science, College of Basic and Applied Sciences, Mountain Top University, Makogi Oba, Nigeria.
ScientificWorldJournal. 2020 May 15;2020:1036364. doi: 10.1155/2020/1036364. eCollection 2020.
Diabetes affects the homeostasis of the circulatory system. Benth S. Moore (Asteraceae) is an edible plant locally used in the treatment of wounds, stomach ulcer, and skin-related conditions in Africa and some other parts of the world. This study investigated the effects of leaf methanol extract and fractions on blood coagulation profile of diabetic Wistar rats. The effect of 100 mg/kg body weight of the methanol extract and partitioned fractions of on blood coagulation profile of STZ-induced diabetic rats were initially evaluated, while graded concentrations (50-200 mg/kg body weight) of the aqueous and hexane fractions were further tested in diabetic rats against standard drugs aspirin (anticoagulant) and metformin (antidiabetic). Rats were allocated into groups ( = 6) and administration was done orally, once daily for 2 weeks. The methanol extract and fractions of at concentrations of 50, 100, and 200 mg/kg significantly prolonged the bleeding (58-200%), clotting (65-133%), prothrombin (176-441%), and activated partial thromboplastin (209-518%) times in diabetic rats compared to the control rats (LD ≥ 5000 mg/kg). Highest prolongation effects were recorded in the diabetic group treated with 100 mg/kg body weight of the hexane fraction. Plasma calcium concentration and platelet counts of treated diabetic rats were significantly ( < 0.05) reduced compared to diabetic control rats, while the red blood cells (RBC), hemoglobin concentration, and packed cell volume (PCV) were significantly increased. This study showed that possess anticoagulant and antianemic activities. The leaves can thus be a potential source of novel anticoagulant and nutraceutical for management of the thrombotic disorder in diabetes and other diseased states.
糖尿病会影响循环系统的内稳态。Benth S. Moore（菊科）是一种可食用的植物，在非洲和世界其他一些地区，当地人用它来治疗伤口、胃溃疡和皮肤疾病。本研究调查了叶甲醇提取物和馏分对糖尿病 Wistar 大鼠凝血谱的影响。首先评估了 100mg/kg 体重甲醇提取物和 STZ 诱导的糖尿病大鼠部分馏分对凝血谱的影响，然后在糖尿病大鼠中进一步测试了水相和己烷相的分级浓度（50-200mg/kg 体重），与标准药物阿司匹林（抗凝剂）和二甲双胍（抗糖尿病药）相对比。大鼠被分配到不同的组（n=6），并每天口服给药一次，持续 2 周。与对照组大鼠相比，甲醇提取物和馏分在 50、100 和 200mg/kg 浓度下可显著延长糖尿病大鼠的出血（58-200%）、凝血（65-133%）、凝血酶原（176-441%）和活化部分凝血活酶时间（209-518%）（LD≥5000mg/kg）。在糖尿病组中，用 100mg/kg 体重的己烷馏分治疗时，记录到的最高延长效果。与糖尿病对照组大鼠相比，用 治疗的糖尿病大鼠的血浆钙浓度和血小板计数显著降低（<0.05），而红细胞（RBC）、血红蛋白浓度和红细胞压积（PCV）显著增加。本研究表明， 具有抗凝和抗贫血活性。因此，该叶片可能是一种新型抗凝和营养保健品的潜在来源，可用于治疗糖尿病和其他疾病状态中的血栓形成障碍。
