Iheagwam Franklyn Nonso, Garuba Precious Amaneshi, Ogunlana Olubanke Olujoke, Chinedu Shalom Nwodo
Department of Biochemistry, Covenant University, PMB 1023, Ota, Ogun State, Nigeria.
Covenant University Public Health and Wellbeing Research Cluster, Covenant University, PMB 1023, Ota, Ogun State, Nigeria.
Vet World. 2023 Aug;16(8):1593-1599. doi: 10.14202/vetworld.2023.1593-1599. Epub 2023 Aug 11.
Diabetes mellitus is a leading cause of mortality worldwide associated with hyperglycemia-induced hematological aberrations and thromboembolic complications. This study aimed to explore the modulatory effect of leaf aqueous crude extract (TCLE) on hematological and coagulation disturbances in a Type 2 diabetic rat model.
High-fat diet streptozotocin-induced diabetic rats were treated orally with 400 and 800 mg/kg body weight TCLE daily for 28 days. Full blood count, coagulation parameters, plasma calcium (Ca), and erythrocyte glycogen (GLYC) levels were assessed using standard procedures.
leaf aqueous crude extract treatment had a significant (p < 0.05) prolonging effect on clotting and bleeding times while increasing Ca, GLYC and mean corpuscular volume in diabetic rats. On the other hand, lymphocytes (LYM), platelet (PLT) count, mean PLT volume, neutrophil-LYM ratio (NLR), and PLT-LYM ratio (PLR) of TCLE-treated diabetic animals were significantly reduced (p < 0.05) compared with untreated diabetic animals. Lymphocyte, PLT count, NLR, and PLR correlated positively (p < 0.05) with plasma glucose, while a significant positive association was observed between Ca and GLYC. On the other hand, a strong negative association (p < 0.05) was observed between clotting time and fasting plasma glucose.
These findings suggest that leaf extract may be useful in reversing diabetic-mediated hematological anomalies due to its anticoagulant and anti-anemic activities.
糖尿病是全球主要的死亡原因之一，与高血糖引起的血液学异常和血栓栓塞并发症相关。本研究旨在探讨叶水粗提物（TCLE）对2型糖尿病大鼠模型血液学和凝血紊乱的调节作用。
高脂饮食链脲佐菌素诱导的糖尿病大鼠，每天口服400和800mg/kg体重的TCLE，持续28天。使用标准程序评估全血细胞计数、凝血参数、血浆钙（Ca）和红细胞糖原（GLYC）水平。
叶水粗提物处理对糖尿病大鼠的凝血和出血时间有显著（p<0.05）的延长作用，同时增加了Ca、GLYC和平均红细胞体积。另一方面，与未处理的糖尿病动物相比，经TCLE处理的糖尿病动物的淋巴细胞（LYM）、血小板（PLT）计数、平均血小板体积、中性粒细胞-淋巴细胞比值（NLR）和血小板-淋巴细胞比值（PLR）显著降低（p<0.05）。淋巴细胞、PLT计数、NLR和PLR与血浆葡萄糖呈正相关（p<0.05），而Ca和GLYC之间观察到显著的正相关。另一方面，凝血时间与空腹血糖之间观察到强烈的负相关（p<0.05）。
这些发现表明，叶提取物可能因其抗凝和抗贫血活性而有助于逆转糖尿病介导的血液学异常。
