Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.
Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji 831100, China.
Int J Mol Sci. 2023 Oct 23;24(20):15477. doi: 10.3390/ijms242015477.
-induced pear Valsa canker is among the most prevalent diseases to impact pear quality and yields. Biocontrol strategies to control plant disease represent an attractive alternative to the application of fungicides. In this study, the potential utility of strain HF1 was assessed as a biocontrol agent against pear Valsa canker. Strain HF1 suppressed mycelium growth by 61.20% and induced the development of malformed hyphae. Both culture filtrate and volatile organic compounds (VOCs) derived from strain HF1 were able to antagonize growth. Treatment with strain HF1-derived culture filtrate or VOCs also induced the destruction of hyphal cell membranes. Headspace mixtures prepared from strain HF1 were analyzed, leading to the identification of 27 potential VOCs. Of the thirteen pure chemicals tested, iberverin, hexanoic acid, and 2-methylvaleraldehyde exhibited the strongest antifungal effects on , with respective EC values of 0.30, 6.65, and 74.07 μL L. Fumigation treatment of pear twigs with each of these three compounds was also sufficient to prevent the development of pear Valsa canker. As such, these results demonstrate that strain HF1 and the volatile compounds iberverin, hexanoic acid, and 2-methylvaleraldehyde exhibit promise as novel candidate biocontrol agents against pear Valsa canker.
诱导性梨腐烂溃疡病是影响梨品质和产量的最普遍病害之一。采用生物防治策略来控制植物病害是替代杀菌剂应用的一种有吸引力的选择。在这项研究中，评估了菌株 HF1 作为防治梨腐烂溃疡病的生物防治剂的潜力。菌株 HF1 通过抑制菌丝生长 61.20%并诱导畸形菌丝的发育来发挥作用。菌株 HF1 的培养滤液和挥发性有机化合物（VOCs）均能拮抗生长。用菌株 HF1 衍生的培养滤液或 VOCs 处理也能诱导菌丝细胞膜的破坏。对菌株 HF1 制备的顶空混合物进行了分析，鉴定出了 27 种潜在的 VOCs。在测试的 13 种纯化学品中，异长春花碱、己酸和 2-甲基戊醛对表现出最强的抗真菌作用，相应的 EC 值分别为 0.30、6.65 和 74.07 μL/L。这三种化合物对梨嫩枝的熏蒸处理也足以防止梨腐烂溃疡病的发生。因此，这些结果表明，菌株 HF1 和挥发性化合物异长春花碱、己酸和 2-甲基戊醛有望成为防治梨腐烂溃疡病的新型候选生物防治剂。
