Asghari-Paskiabi Farnoush, Imani Mohammad, Eybpoosh Sana, Rafii-Tabar Hashem, Razzaghi-Abyaneh Mehdi
Department of Medical Physics and Biomedical Engineering, School of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran.
Department of Mycology, Pasteur Institute of Iran, Tehran, Iran.
Front Microbiol. 2020 May 21;11:1019. doi: 10.3389/fmicb.2020.01019. eCollection 2020.
Biosynthesis of nanoparticles (NPs) by microorganisms is a cost- and energy-effective approach. However, how the production of NPs affects the population of producing organism remains as an unresolved question. The present study aimed to evaluate the kinetics of growth in relation to synthesis of selenium sulfide nanoparticles by using a population model. To this end, the population of cells was investigated in terms of colony forming units (CFU) in the presence of the substrate in different time points. Fluctuation of sulfite reductase (SiR) activity, expression of and genes, and concentrations of sulfite and selenium were evaluated to support the population findings. CFU values in the test groups were lower than those in the control counterparts. The rise and fall of the SiR activity and and gene expression conformed to the variations of CFU values. The rate of reduction in the selenium and sulfite concentrations tended to decrease over the time. In conclusion, the cells population was negatively and positively affected by selenium and sulfite concentrations, respectively. The indirect relationship of the selenium ions concentration in the path analysis revealed that the product, selenium sulfide nanoparticles, caused this drop in cells population.
微生物合成纳米颗粒（NPs）是一种经济高效的方法。然而，纳米颗粒的产生如何影响生产微生物的数量仍是一个未解决的问题。本研究旨在通过使用种群模型评估与硫化硒纳米颗粒合成相关的生长动力学。为此，在不同时间点的底物存在下，根据菌落形成单位（CFU）对细胞数量进行了研究。评估了亚硫酸盐还原酶（SiR）活性、相关基因的表达以及亚硫酸盐和硒的浓度波动，以支持关于细胞数量的研究结果。测试组中的CFU值低于对照组。SiR活性以及相关基因表达的上升和下降与CFU值的变化一致。随着时间的推移，硒和亚硫酸盐浓度的降低速率趋于下降。总之，细胞数量分别受到硒和亚硫酸盐浓度的负面影响和正面影响。路径分析中硒离子浓度的间接关系表明，产物硫化硒纳米颗粒导致了细胞数量的下降。
