Ahmad Maged Sayed, Yasser Manal Mohamed, Sholkamy Essam Nageh, Ali Ali Mohamed, Mehanni Magda Mohamed
Department of Botany, Faculty of Science, University of Beni-Suef, Beni-Suef, Egypt.
Department of Botany, Faculty of Science, University of Beni-Suef, Beni-Suef, Egypt ; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Int J Nanomedicine. 2015 May 6;10:3389-401. doi: 10.2147/IJN.S82707. eCollection 2015.
Selenium is an important component of human diet and a number of studies have declared its chemopreventive and therapeutic properties against cancer. However, very limited studies have been conducted about the properties of selenium nanostructured materials in comparison to other well-studied selenospecies. Here, we have shown that the anticancer property of biostabilized selenium nanorods (SeNrs) synthesized by applying a novel strain Ess_amA-1 of Streptomyces bikiniensis. The strain was grown aerobically with selenium dioxide and produced stable SeNrs with average particle size of 17 nm. The optical, structural, morphological, elemental, and functional characterizations of the SeNrs were carried out using techniques such as UV-vis spectrophotometry, transmission electron microscopy, energy dispersive X-ray spectrometry, and Fourier transform infrared spectrophotometry, respectively. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay revealed that the biosynthesized SeNrs induces cell death of Hep-G2 and MCF-7 human cancer cells. The lethal dose (LD50%) of SeNrs on Hep-G2 and MCF-7 cells was recorded at 75.96 μg/mL and 61.86 μg/mL, respectively. It can be concluded that S. bikiniensis strain Ess_amA-1 could be used as renewable bioresources of biosynthesis of anticancer SeNrs. A hypothetical mechanism for anticancer activity of SeNrs is also proposed.
硒是人类饮食中的重要成分，许多研究已表明其对癌症具有化学预防和治疗特性。然而，与其他经过充分研究的含硒物种相比，关于硒纳米结构材料特性的研究非常有限。在此，我们展示了由比基尼链霉菌的新型菌株Ess_amA - 1合成的生物稳定化硒纳米棒（SeNrs）的抗癌特性。该菌株在二氧化硒存在下好氧生长，并产生平均粒径为17 nm的稳定SeNrs。分别使用紫外可见分光光度法、透射电子显微镜、能量色散X射线光谱法和傅里叶变换红外分光光度法等技术对SeNrs进行了光学、结构、形态、元素和功能表征。MTT（3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐）分析表明，生物合成的SeNrs可诱导Hep - G2和MCF - 7人癌细胞死亡。SeNrs对Hep - G2和MCF - 7细胞的致死剂量（LD50%）分别记录为75.96 μg/mL和61.86 μg/mL。可以得出结论，比基尼链霉菌菌株Ess_amA - 1可作为抗癌SeNrs生物合成的可再生生物资源。还提出了SeNrs抗癌活性的假设机制。
