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饥饿成纤维细胞培养上清液对用癌干细胞（LA7）处理的免疫抑制大鼠的全身影响。

Systemic effects of starved fibroblast culture supernatant on immunosuppressed rats treated with cancer stem cells (LA7).

作者信息

Pourbagher Roghayeh, Feizi Farideh, Akhavan Niaki Haleh, Sabour Davood, Zabihi Ebrahim, Ghorbani Hossein, Gooran Sahar, Abedian Zeinab, Majidi Fatemeh, Mostafazadeh Amrollah

机构信息

Student Research Committee, Babol University of Medical Sciences, Babol, Iran.

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

出版信息

Caspian J Intern Med. 2020;11(2):135-142. doi: 10.22088/cjim.11.2.135.

DOI:10.22088/cjim.11.2.135
PMID:32509240
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7265514/
Abstract

BACKGROUND

The present study aimed to investigate and compare the effect of starved fibroblast culture supernatant (SFS), DMEM and normal saline alone or along with LA7 on dexamethasone-treated immunosuppressed Wistar rats.

METHODS

After the isolation of fibroblasts from the fresh foreskin of children, it was cultured in serum-free DMEM, and the supernatant collected after 16 hours (16h-SFS). This solution and the other treatments were injected subcutaneously into the rats from each group once daily for 14 days. The liver, intestine and lung histology along with blood cellular and biochemical characteristics were studied.

RESULTS

The results showed that dexamethasone as immunosuppressant reduced the body weight. The histological change in the liver was mild fibrosis induced by LA7+16h-SFS. Also, among the different blood cellular and biochemical indices measured, the eosinophil percentage in the 16h-SFS treated rats , glucose levels in the 16h-SFS+LA7 group and triglyceride concentrations in the 16h-SFS group were changed (p<0.05).

CONCLUSION

This study showed that the secretions of starved fibroblasts especially that combined with LA7 cancer stem cells could induce some minor histological and biochemical changes in immunosuppressed rats, and also it opened a new window for subsequent investigations on unknown mechanisms related to this work.

摘要

背景

本研究旨在调查和比较饥饿成纤维细胞培养上清液（SFS）、DMEM和生理盐水单独或与LA7联合使用对经地塞米松处理的免疫抑制Wistar大鼠的影响。

方法

从儿童新鲜包皮中分离出成纤维细胞后，将其在无血清DMEM中培养，并在16小时后收集上清液（16小时-SFS）。将该溶液和其他处理方法每天皮下注射到每组大鼠体内，持续14天。研究了肝脏、肠道和肺的组织学以及血细胞和生化特征。

结果

结果表明，作为免疫抑制剂的地塞米松降低了体重。LA7 + 16小时-SFS诱导肝脏出现轻度纤维化组织学变化。此外，在测量的不同血细胞和生化指标中，16小时-SFS处理的大鼠中的嗜酸性粒细胞百分比、16小时-SFS + LA7组中的葡萄糖水平以及16小时-SFS组中的甘油三酯浓度发生了变化（p < 0.05）。

结论

本研究表明，饥饿成纤维细胞的分泌物，尤其是与LA7癌症干细胞结合的分泌物，可在免疫抑制大鼠中诱导一些轻微的组织学和生化变化，并且为后续关于与此工作相关的未知机制的研究打开了一扇新窗口。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c1/7265514/d60c66fb2901/cjim-11-135-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c1/7265514/bd6816e7924b/cjim-11-135-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c1/7265514/cbe5c78e1444/cjim-11-135-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c1/7265514/011e21b23a89/cjim-11-135-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c1/7265514/31d4bcaf058e/cjim-11-135-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c1/7265514/e4fe8aa37e1f/cjim-11-135-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c1/7265514/d60c66fb2901/cjim-11-135-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c1/7265514/bd6816e7924b/cjim-11-135-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c1/7265514/cbe5c78e1444/cjim-11-135-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c1/7265514/011e21b23a89/cjim-11-135-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c1/7265514/31d4bcaf058e/cjim-11-135-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c1/7265514/e4fe8aa37e1f/cjim-11-135-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c1/7265514/d60c66fb2901/cjim-11-135-g006.jpg

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本文引用的文献

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