Khayi Slimane, Azza Nour Elhouda, Gaboun Fatima, Pirro Stacy, Badad Oussama, Claros M Gonzalo, Lightfoot David A, Unver Turgay, Chaouni Bouchra, Merrouch Redouane, Rahim Bouchra, Essayeh Soumaya, Ganoudi Matika, Abdelwahd Rabha, Diria Ghizlane, Mdarhi Meriem Alaoui, Labhilili Mustapha, Iraqi Driss, Mouhaddab Jamila, Sedrati Hayat, Memari Majid, Hamamouch Noureddine, Alché Juan de Dios, Boukhatem Noureddine, Mrabet Rachid, Dahan Rachid, Legssyer Adelkhaleq, Khalfaoui Mohamed, Badraoui Mohamed, Van de Peer Yves, Tatusova Tatiana, El Mousadik Abdelhamid, Mentag Rachid, Ghazal Hassan
Biotechnology Unit, National Institute of Agricultural Research (INRA), Rabat, Morocco, Morocco.
Laboratory of Biotechnology and Valorization of Natural Resources (LBVRN), Faculty of Sciences, University Ibn Zohr, Agadir, Morocco.
F1000Res. 2018 Aug 17;7:1310. doi: 10.12688/f1000research.15719.2. eCollection 2018.
The Argane tree ( L. Skeels) is an endemic tree of mid-western Morocco that plays an important socioeconomic and ecologic role for a dense human population in an arid zone. Several studies confirmed the importance of this species as a food and feed source and as a resource for both pharmaceutical and cosmetic compounds. Unfortunately, the argane tree ecosystem is facing significant threats from environmental changes (global warming, over-population) and over-exploitation. Limited research has been conducted, however, on argane tree genetics and genomics, which hinders its conservation and genetic improvement. Here, we present a draft genome assembly of . A reliable reference genome of was created using a hybrid assembly approach combining short and long sequencing reads. In total, 144 Gb Illumina HiSeq reads and 7.6 Gb PacBio reads were produced and assembled. The final draft genome comprises 75 327 scaffolds totaling 671 Mb with an N50 of 49 916 kb. The draft assembly is close to the genome size estimated by -mers distribution and covers 89% of complete and 4.3 % of partial orthologous groups in BUSCO. The genome will be useful for assessing biodiversity leading to efficient conservation of this endangered endemic tree. Furthermore, the genome may enable genome-assisted cultivar breeding, and provide a better understanding of important metabolic pathways and their underlying genes for both cosmetic and pharmacological.
阿甘树（L. Skeels）是摩洛哥中西部的一种特有树种，在干旱地区对密集的人口起着重要的社会经济和生态作用。多项研究证实了该物种作为食物和饲料来源以及作为制药和化妆品化合物资源的重要性。不幸的是，阿甘树生态系统正面临着来自环境变化（全球变暖、人口过剩）和过度开发的重大威胁。然而，关于阿甘树遗传学和基因组学的研究有限，这阻碍了其保护和遗传改良。在此，我们展示了[具体物种名称]的基因组组装草图。利用结合短读长和长读长测序的混合组装方法创建了[具体物种名称]可靠的参考基因组。总共产生并组装了144 Gb的Illumina HiSeq读长和7.6 Gb的PacBio读长。最终的基因组草图包含75327个支架，总计671 Mb，N50为49916 kb。该组装草图接近通过k-mer分布估计的基因组大小，覆盖了BUSCO中89%的完整直系同源组和4.3%的部分直系同源组。[具体物种名称]的基因组将有助于评估生物多样性，从而有效地保护这种濒危的特有树种。此外，该基因组可能有助于基因组辅助品种育种，并更好地理解化妆品和药理学方面重要的代谢途径及其潜在基因。
