Khayi Slimane, Gaboun Fatima, Pirro Stacy, Tatusova Tatiana, El Mousadik Abdelhamid, Ghazal Hassan, Mentag Rachid
CRRA-Rabat, National Institute for Agricultural Research (INRA), Rabat 10101, Morocco.
Iridian Genomes, Inc., Bethesda, MD 20817, USA.
Plants (Basel). 2020 Oct 13;9(10):1354. doi: 10.3390/plants9101354.
(Sapotaceae), an important endemic Moroccan oil tree, is a primary source of argan oil, which has numerous dietary and medicinal proprieties. The plant species occupies the mid-western part of Morocco and provides great environmental and socioeconomic benefits. The complete chloroplast (cp) genome of was sequenced, assembled, and analyzed in comparison with those of two Sapotaceae members. The cp genome is 158,848 bp long, with an average GC content of 36.8%. The cp genome exhibits a typical quadripartite and circular structure consisting of a pair of inverted regions (IR) of 25,945 bp in length separating small single-copy (SSC) and large single-copy (LSC) regions of 18,591 and 88,367 bp, respectively. The annotation of cp genome predicted 130 genes, including 85 protein-coding genes (CDS), 8 ribosomal RNA (rRNA) genes, and 37 transfer RNA (tRNA) genes. A total of 44 long repeats and 88 simple sequence repeats (SSR) divided into mononucleotides (76), dinucleotides (7), trinucleotides (3), tetranucleotides (1), and hexanucleotides (1) were identified in the cp genome. Phylogenetic analyses using the maximum likelihood (ML) method were performed based on 69 protein-coding genes from 11 species of . The results confirmed the close position of to the genus, supporting the revisiting of its taxonomic status. The complete chloroplast genome sequence will be valuable for further studies on the conservation and breeding of this medicinally and culinary important species and also contribute to clarifying the phylogenetic position of the species within Sapotaceae.
摩洛哥坚果树（山榄科）是摩洛哥一种重要的本土产油树，是阿甘油的主要来源，阿甘油具有多种饮食和药用特性。该植物物种分布在摩洛哥中西部地区，带来了巨大的环境和社会经济效益。对摩洛哥坚果树的完整叶绿体（cp）基因组进行了测序、组装，并与两种山榄科植物的叶绿体基因组进行了比较分析。摩洛哥坚果树的cp基因组长度为158,848 bp，平均GC含量为36.8%。该cp基因组呈现出典型的四分体环状结构，由一对长度为25,945 bp的反向重复序列（IR）分隔开，分别为长度18,591 bp的小单拷贝（SSC）区域和长度88,367 bp的大单拷贝（LSC）区域。摩洛哥坚果树cp基因组的注释预测有130个基因，包括85个蛋白质编码基因（CDS）、8个核糖体RNA（rRNA）基因和37个转运RNA（tRNA）基因。在摩洛哥坚果树的cp基因组中总共鉴定出44个长重复序列和88个简单序列重复序列（SSR），分为单核苷酸重复序列（76个）、二核苷酸重复序列（7个）、三核苷酸重复序列（3个）、四核苷酸重复序列（1个）和六核苷酸重复序列（1个）。基于来自11种山榄科植物的69个蛋白质编码基因，采用最大似然法（ML）进行了系统发育分析。结果证实了摩洛哥坚果树与牛油果属的亲缘关系较近，支持重新审视其分类地位。完整的叶绿体基因组序列对于进一步研究这种具有药用和烹饪价值的重要物种的保护和育种将具有重要价值，也有助于阐明该物种在山榄科中的系统发育位置。
