Lulan Theodore Y K, Fatmawati Sri, Santoso Mardi, Ersam Taslim
Department of Chemistry, Faculty of Science, Institut Teknologi Sepuluh Nopember, Surabaya, 60111, Indonesia.
Department of Chemistry, Faculty of Science and Engineering, University of Nusa Cendana, Kupang, 85000, Indonesia.
Heliyon. 2020 May 30;6(5):e04102. doi: 10.1016/j.heliyon.2020.e04102. eCollection 2020 May.
Over the past few decades, complementary medicine therapy using medicinal plants have been developed in healthcare. Phytochemical studies about medicinal plants have been conducted to verify their potency as medicinal remedies in modern therapeutics. commonly known as Meranti Jawa in Indonesia is traditionally used to treat diseases such as diarrhea, diabetic and malaria. This study aimed to isolate bioactive compounds from using bioguided fractionation method. The bioactivity measured were antioxidant, antidiabetic, and antiplasmodial activity. -glucosidase and -amylase assays were applied to estimate the antidiabetic activity of . The antioxidant activities were determined by using the free radical scavenging assays 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2-2″-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Analysis of total flavonoid and phenolic contents were expressed as Quercetin Equivalent (QE) and Gallic Acid Equivalent (GAE), respectively. The i antiplasmodial activity test of methanol extract of was also conducted against strain 3D7. Purification of the ethyl acetate fraction of the methanol extract of resulted in an oligostilbenes namely α-viniferin (). The structure of the α-viniferin was characterized by comprehensive spectral analysis including IR, 1D and 2D NMR, and in comparison with the literature data. Compound showed an a-glucosidase and -amylase inhibitory activity with IC values of 256.17 and 212.79 μg/mL, respectively. The antiplasmodial activity test against strain 3D7 at a concentration of 100 μg/mL revealed a strong antiplasmodial inhibitory activity with IC value of 2.76 μg/mL. Our findings indicated that α-viniferin () which is isolated from extract could be regarded as potential antidiabetic and antiplasmodial resources in the future.
在过去几十年中，医疗保健领域已开发出使用药用植物的补充医学疗法。人们对药用植物进行了植物化学研究，以验证它们在现代治疗中作为药物的效力。在印度尼西亚通常被称为爪哇柳桉木的这种植物，传统上用于治疗腹泻、糖尿病和疟疾等疾病。本研究旨在采用生物导向分级分离法从该植物中分离生物活性化合物。所测定的生物活性为抗氧化、抗糖尿病和抗疟活性。应用α-葡萄糖苷酶和α-淀粉酶测定法来评估该植物的抗糖尿病活性。通过自由基清除测定法1,1-二苯基-2-苦基肼（DPPH）和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）来测定抗氧化活性。总黄酮和酚类含量的分析分别以槲皮素当量（QE）和没食子酸当量（GAE）表示。还对该植物甲醇提取物针对3D7菌株进行了抗疟活性测试。该植物甲醇提取物的乙酸乙酯部分的纯化得到一种低聚芪类化合物，即α-葡萄素（）。通过包括红外光谱、一维和二维核磁共振在内的综合光谱分析，并与文献数据进行比较，对α-葡萄素的结构进行了表征。化合物显示出α-葡萄糖苷酶和α-淀粉酶抑制活性，IC值分别为256.17和212.79μg/mL。在浓度为100μg/mL时针对3D7菌株的抗疟活性测试显示出较强的抗疟抑制活性，IC值为2.76μg/mL。我们的研究结果表明，从该植物提取物中分离出的α-葡萄素（）未来可被视为潜在的抗糖尿病和抗疟资源。
