Thao Nguyen Phuong, Binh Pham Thanh, Luyen Nguyen Thi, Hung Ta Manh, Dang Nguyen Hai, Dat Nguyen Tien
Advanced Center for Bio-Organic Chemistry, Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
Graduate University of Science and Technology, VAST, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
J Anal Methods Chem. 2018 May 9;2018:2794904. doi: 10.1155/2018/2794904. eCollection 2018.
As part of an ongoing search for new natural products from medicinal plants to treat type 2 diabetes, two new compounds, a megastigmane sesquiterpenoid sulfonic acid () and a new cyclohexylethanoid derivative (), and seven related known compounds () were isolated from the leaves of (Osbeck.) Merr. The structures of the compounds were conducted via interpretation of their spectroscopic data (1D and 2D NMR, IR, and MS), and the absolute configurations of compound were determined by the modified Mosher's method. The MeOH extract of was found to inhibit -amylase and -glucosidase inhibitory activities as well as by the compounds isolated from this extract. Furthermore, compound showed the strongest effect with IC values of 112.8 ± 15.1 g/mL (against -amylase) and 785.9 ± 12.7 g/mL (against -glucosidase). Compounds , , and showed moderate -amylase and -glucosidase inhibitory effects. Other compounds showed weak or did not show any effect on both enzymes. The results suggested that the antidiabetic properties from the leaves of are not simply a result of each isolated compound but are due to other components such as the accessibility of polyphenolic groups to -amylase and -glucosidase activities.
作为从药用植物中寻找治疗2型糖尿病新天然产物的一项正在进行的研究的一部分，从楤木（Osbeck.）Merr.的叶子中分离出两种新化合物，一种巨大戟烷倍半萜磺酸（）和一种新的环己醇衍生物（），以及七种相关的已知化合物（）。通过对它们的光谱数据（一维和二维核磁共振、红外光谱和质谱）的解释确定了这些化合物的结构，并且通过改良的莫舍尔方法确定了化合物的绝对构型。发现楤木的甲醇提取物以及从该提取物中分离出的化合物具有抑制α-淀粉酶和α-葡萄糖苷酶的活性。此外，化合物显示出最强的效果，对α-淀粉酶的IC值为112.8±15.1μg/mL，对α-葡萄糖苷酶的IC值为785.9±12.7μg/mL。化合物、和显示出中等程度的α-淀粉酶和α-葡萄糖苷酶抑制作用。其他化合物对这两种酶显示出微弱的作用或没有任何作用。结果表明，楤木叶的抗糖尿病特性不仅仅是每种分离化合物的结果，而是由于其他成分，例如多酚基团对α-淀粉酶和α-葡萄糖苷酶活性的可及性。
