Piessens W F, Remold H G, David J R
J Immunol. 1977 Jun;118(6):2078-82.
Guinea pig macrophages pretreated with the esterase inhibitor, antithrombin III (AT III) show increased responsiveness to macrophage-activating factor (MAF) as demonstrated by their enhanced cytotoxicity for tumor cells. Other proteins that are not esterase inhibitors did not enhance the effect of MAF on the macrophage. Enhancement of MAF activity was also obtained when macrophages were preincubated with the cell surface reactant, diazotized sulfanilic acid (DSA). These studies indicate that the effect of MAF can be enhanced by chemical modifications of the macrophage membrane. They also provide further evidence to support the hypothesis that an esterase on the macrophage membrane modulates this cell's responsiveness to lymphocyte mediators.
用酯酶抑制剂抗凝血酶III（AT III）预处理的豚鼠巨噬细胞，对巨噬细胞活化因子（MAF）的反应性增强，这表现为它们对肿瘤细胞的细胞毒性增强。其他非酯酶抑制剂的蛋白质并未增强MAF对巨噬细胞的作用。当巨噬细胞与细胞表面反应物重氮化磺胺酸（DSA）预孵育时，也能增强MAF活性。这些研究表明，通过巨噬细胞膜的化学修饰可以增强MAF的作用。它们还提供了进一步的证据来支持这一假说，即巨噬细胞膜上的一种酯酶调节该细胞对淋巴细胞介质的反应性。
