Sober A J, Haynie M, David J R
J Immunol. 1979 May;122(5):1731-5.
The enhanced uptake and incorporation of 3H-glucosamine by guinea pig peritoneal exudate macrophages stimulated by macrophage-activating factor (MAF) were studied. MAF was produced by concanavalin A- (Con A) stimulated lymphocytes. Two systems were developed to assay this phenomenon, one using microtiter plates, the other using suspension cultures. Enhanced uptake of 3H-glucosamine by macrophages was found after 48-hr incubation in microtiter plates and after 24 hr in suspension cultures. Increased uptake of 14C-glucose and 3H-galactose was also observed under these conditions. The enhanced uptake of 3H-glucosamine was markedly reduced or abolished by the addition of glucose to the cell cultures or by replacement of the medium with fresh medium before pulsing with labeled sugar. These observations indicate that the mechanism of enhanced uptake reflects in part increased glucose utilization by the stimulated macrophages.
研究了巨噬细胞激活因子（MAF）刺激的豚鼠腹腔渗出巨噬细胞对3H-葡萄糖胺摄取和掺入的增强情况。MAF由伴刀豆球蛋白A（Con A）刺激的淋巴细胞产生。开发了两种系统来检测这一现象，一种使用微量滴定板，另一种使用悬浮培养。在微量滴定板中孵育48小时后以及在悬浮培养中孵育24小时后，发现巨噬细胞对3H-葡萄糖胺的摄取增强。在这些条件下，还观察到14C-葡萄糖和3H-半乳糖的摄取增加。在细胞培养物中添加葡萄糖或在用标记糖脉冲之前用新鲜培养基替换培养基，可使3H-葡萄糖胺的摄取增强明显降低或消除。这些观察结果表明，摄取增强的机制部分反映了受刺激巨噬细胞葡萄糖利用的增加。
