Schneider P B
J Lipid Res. 1977 May;18(3):396-9.
A sensitive radioenzymatic assay for glycerol and acylglycerols is described. The assay depends on the quantitative phosphorylation of glycerol to glycerophosphate by glycerol kinase using [gamma-32P]ATP as a substrate. The 32P content of the formed glycerophosphate is determined and gives a measure of the original glycerol content. Acylglycerols can be determined by prior hydrolysis to glycerol. The assay is sensitive to about 0.1 nmol of glycerol and can be extended to 100 nmol. The assay can be applied to the determination of acylglycerols separated by thin-layer chromatography in amounts as low as 0.5 nmol. The assay is particularly useful in the determination of the specific activity of 14C- or 3H-labeled glycerol moeities.
本文描述了一种用于甘油和酰基甘油的灵敏放射酶测定法。该测定法依赖于甘油激酶以[γ-32P]ATP为底物将甘油定量磷酸化为甘油磷酸。测定所形成的甘油磷酸中的32P含量，可得出原始甘油含量的测量值。酰基甘油可通过预先水解为甘油来测定。该测定法对约0.1 nmol甘油敏感，可扩展至100 nmol。该测定法可用于测定通过薄层色谱分离的低至0.5 nmol的酰基甘油。该测定法在测定14C或3H标记的甘油部分的比活度时特别有用。
