Pelech S L, Pritchard P H, Brindley D N, Vance D E
Biochem J. 1983 Oct 15;216(1):129-36. doi: 10.1042/bj2160129.
The influence of cyclic AMP analogues and fatty acids on glycerolipid biosynthesis in monolayer cultures of rat hepatocytes was investigated. Chlorophenylthio-cyclic AMP and adenosine 3':5'-cyclic phosphorothioate inhibited the rate of triacylglycerol synthesis from [1(3)-3H]glycerol, and phosphatidylcholine synthesis from [Me-3H]-choline. Supplementation of the hepatocytes with palmitate (1 mM) reversed chlorophenylthio-cyclic AMP inhibition of triacylglycerol synthesis. Similarly, cyclic AMP analogue-inhibition of phosphatidylcholine synthesis was abolished when the cells were simultaneously incubated with oleate (3 mM). Reactivation of phosphatidylcholine synthesis in chlorophenylthio-cyclic AMP-supplemented cells with oleate was accompanied by conversion of CTP: phosphocholine cytidylyltransferase into the membrane-bound form, since these cells released the enzyme more slowly after treatment with digitonin. The opposing actions of cyclic AMP and fatty acids are discussed in relation to the regulation of glycerolipid biosynthesis during starvation, diabetes and stress.
研究了环磷酸腺苷类似物和脂肪酸对大鼠肝细胞单层培养物中甘油脂质生物合成的影响。氯苯硫基环磷酸腺苷和腺苷3':5'-环硫代磷酸酯抑制了[1(3)-3H]甘油合成三酰甘油的速率，以及[甲基-3H]胆碱合成磷脂酰胆碱的速率。用棕榈酸（1 mM）补充肝细胞可逆转氯苯硫基环磷酸腺苷对三酰甘油合成的抑制作用。同样，当细胞同时与油酸（3 mM）孵育时，环磷酸腺苷类似物对磷脂酰胆碱合成的抑制作用被消除。用油酸使补充了氯苯硫基环磷酸腺苷的细胞中的磷脂酰胆碱合成重新激活，伴随着CTP：磷酸胆碱胞苷转移酶转变为膜结合形式，因为这些细胞在用洋地黄皂苷处理后释放该酶的速度更慢。讨论了环磷酸腺苷和脂肪酸的相反作用与饥饿、糖尿病和应激期间甘油脂质生物合成调节的关系。
